About: Restoring wild-type conformation and DNA-binding activity of mutant p53 is insufficient for restoration of transcriptional activity

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About: Restoring wild-type conformation and DNA-binding activity of mutant p53 is insufficient for restoration of transcriptional activity Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Most human tumors contain inactivated p53 protein, either by mutations and/or functional deactivation. Restoration of wild-type p53 function could be one of the key tools in new anticancer therapy. Using an electromobility shift assay, we investigated the effect of temperature on DNA binding of wild-type and mutant p53 proteins. We showed that analysis of the DNA-binding capacity of mutant p53 proteins is complicated by the temperature at which the assay is performed. Furthermore, neither ability to bind to DNA nor con-formational analysis accurately defines the transcriptional activity of human tumor-derived p53 mutant proteins. That some mutants can bind DNA and adopt a wildtype conformation in vitro, but are transcriptionally inactive in vivo, points to the involvement of cellular factors required for transactivation. Therefore, the common use of purified proteins and in vitro determinations of DNA binding and conformation are not the best indicators of the functional properties of mutant p53 Most human tumors contain inactivated p53 protein, either by mutations and/or functional deactivation. Restoration of wild-type p53 function could be one of the key tools in new anticancer therapy. Using an electromobility shift assay, we investigated the effect of temperature on DNA binding of wild-type and mutant p53 proteins. We showed that analysis of the DNA-binding capacity of mutant p53 proteins is complicated by the temperature at which the assay is performed. Furthermore, neither ability to bind to DNA nor con-formational analysis accurately defines the transcriptional activity of human tumor-derived p53 mutant proteins. That some mutants can bind DNA and adopt a wildtype conformation in vitro, but are transcriptionally inactive in vivo, points to the involvement of cellular factors required for transactivation. Therefore, the common use of purified proteins and in vitro determinations of DNA binding and conformation are not the best indicators of the functional properties of mutant p53 (en)
Title	Restoring wild-type conformation and DNA-binding activity of mutant p53 is insufficient for restoration of transcriptional activity Restoring wild-type conformation and DNA-binding activity of mutant p53 is insufficient for restoration of transcriptional activity (en)
skos:prefLabel	Restoring wild-type conformation and DNA-binding activity of mutant p53 is insufficient for restoration of transcriptional activity Restoring wild-type conformation and DNA-binding activity of mutant p53 is insufficient for restoration of transcriptional activity (en)
skos:notation	RIV/00216224:14310/06:00040131!RIV10-MZ0-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/05/0416), P(GP301/04/P025), P(IAA500040513), P(KJB500040502), P(NR8338), Z(AV0Z50040507), Z(MZ0MOU2005)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Müller, Petr Vojtěšek, Bořivoj Brázda, Václav Hrazdilová, Kristýna
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	497421
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/06:00040131
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	p53 mutants; DNA binding; Protein/DNA complex (en)
http://linked.open.../riv/klicoveSlovo	DNA binding Protein/DNA complex p53 mutants
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[D0B260EF7DD5]
http://linked.open...i/riv/nazevZdroje	Biochemical and Biophysical Research Communications
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of novel therapeutic strategies through sensitising tumour cells to anti-cancer drugs by targeting p53-kinases and p53 homologues Functional analysis of the p53-dependent pathways in breast cancer: Integration of genomic and proteomic approaches using DNA-macroarray and SELDI-TOF mass spectrometry techniques Influence of DNA superhelicity on sequence specific and structure selective binding of the p53 protein Wild type and mutant p53 tumor suppressor protein. Intermolecular interactions, changes in conformation and new micro-methods of their analysis Mutants of tumor supressor protein p53 and regulation of thei DNA binding activity
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	351
http://linked.open...iv/tvurceVysledku	Brázda, Václav Vojtěšek, Bořivoj Müller, Petr Hrazdilová, Kristýna
http://linked.open...ain/vavai/riv/wos	000242119200030
http://linked.open...n/vavai/riv/zamer	Biophysics of dynamic structures and functions of biological systems Functional diagnostics of malignant tumours
issn	0006-291X
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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