About: In situ hybridization for mRNA detection in Arabidopsis tissue sections.

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Plant biology is currently confronted with an overflow of expression profile data provided by high-throughput microarray transcription analyses. However, the tissue and cellular resolution of these techniques is limited. Thus, it is still necessary to examine the expression pattern of selected candidate genes at a cellular level. Here we present an in situ mRNA hybridization method that is routinely used in the analysis of gene expression patterns. The protocol is optimized for mRNA localizations in sectioned tissue of Arabidopsis seedlings including embryos, roots, hypocotyls, young primary leaves and flowers. The detailed protocol, recommended controls and troubleshooting are presented along with examples of application. The total time for the process is 10 days. Plant biology is currently confronted with an overflow of expression profile data provided by high-throughput microarray transcription analyses. However, the tissue and cellular resolution of these techniques is limited. Thus, it is still necessary to examine the expression pattern of selected candidate genes at a cellular level. Here we present an in situ mRNA hybridization method that is routinely used in the analysis of gene expression patterns. The protocol is optimized for mRNA localizations in sectioned tissue of Arabidopsis seedlings including embryos, roots, hypocotyls, young primary leaves and flowers. The detailed protocol, recommended controls and troubleshooting are presented along with examples of application. The total time for the process is 10 days. (en)
Title	In situ hybridization for mRNA detection in Arabidopsis tissue sections. In situ hybridization for mRNA detection in Arabidopsis tissue sections. (en)
skos:prefLabel	In situ hybridization for mRNA detection in Arabidopsis tissue sections. In situ hybridization for mRNA detection in Arabidopsis tissue sections. (en)
skos:notation	RIV/00216224:14310/06:00018084!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LC06034), Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Hejátko, Jan Friml, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	479124
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/06:00018084
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	in situ mRNA localization; tissue sections; plants (en)
http://linked.open.../riv/klicoveSlovo	plants in situ mRNA localization tissue sections
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[8B1EB0AE015A]
http://linked.open...i/riv/nazevZdroje	Nature Protocols
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Regulation of morphogenesis of plant cells and organs
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	1
http://linked.open...iv/tvurceVysledku	Friml, Jiří Hejátko, Jan Benková, Eva Heisler, M. Brewer, P.
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
issn	1745-2473
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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