About: High-throughput characterization of enzymes from genomic and proteomic projects-multivariate statistical approach

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About: High-throughput characterization of enzymes from genomic and proteomic projects-multivariate statistical approach Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Hight throughput genomic and proteomic methods release a lot of novel enzymes every year which require systematic characterization and cataloguing of their properties. Here, we illustrate a novel approach, using multivariate statistics to characterize and describe a protein family with broad substrate specificity. Principle of the approach consists in multivariate statistic method, principle component analysis [1], which enabled firstly to choose sufficient set of 30 substrates from 194 halogenated compounds respecting maximum variability in physical-chemical properties [2]. Quick and reliable enzymatic assay follows the selection and produces an activity data of particular proteins with selected substrates. Third step is application of principal component analysis on enzyme activity data matrix to describe the difference in substrate specifities. In paralell, kinetic constants Km and kcat were measured. Hight throughput genomic and proteomic methods release a lot of novel enzymes every year which require systematic characterization and cataloguing of their properties. Here, we illustrate a novel approach, using multivariate statistics to characterize and describe a protein family with broad substrate specificity. Principle of the approach consists in multivariate statistic method, principle component analysis [1], which enabled firstly to choose sufficient set of 30 substrates from 194 halogenated compounds respecting maximum variability in physical-chemical properties [2]. Quick and reliable enzymatic assay follows the selection and produces an activity data of particular proteins with selected substrates. Third step is application of principal component analysis on enzyme activity data matrix to describe the difference in substrate specifities. In paralell, kinetic constants Km and kcat were measured. (en)
Title	High-throughput characterization of enzymes from genomic and proteomic projects-multivariate statistical approach High-throughput characterization of enzymes from genomic and proteomic projects-multivariate statistical approach (en)
skos:prefLabel	High-throughput characterization of enzymes from genomic and proteomic projects-multivariate statistical approach High-throughput characterization of enzymes from genomic and proteomic projects-multivariate statistical approach (en)
skos:notation	RIV/00216224:14310/06:00017056!RIV11-MSM-14310___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021622412)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Prokop, Zbyněk Fořtová, Andrea Damborský, Jiří Chaloupková, Radka Monincová, Marta
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	477734
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/06:00017056
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	haloalkane dehalogenase; substrate specificity; screening; multivariate statistics (en)
http://linked.open.../riv/klicoveSlovo	multivariate statistics screening substrate specificity haloalkane dehalogenase
http://linked.open...ontrolniKodProRIV	[59A71E58AA50]
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2006
http://linked.open...iv/tvurceVysledku	Chaloupková, Radka Damborský, Jiří Fořtová, Andrea Prokop, Zbyněk Sato, Yukari Monincová, Marta
http://linked.open...n/vavai/riv/zamer	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
http://localhost/t...ganizacniJednotka	14310

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