About: Genotypic characterisation of vancomycin-resistant <I>Enterococcus faecium</I> isolates from haemato-oncological patients at Olomouc University Hospital, Czech Republic     Goto   Sponge   NotDistinct   Permalink

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  • This study describes the first molecular characterisation of clinical isolates of vancomycin-resistant enterococci (VRE) in the Czech Republic. Of 2647 patient isolates of Enterococcus spp. from 1997-2002, 121 (4.6%) were identified as VRE. The most common isolates were VanA+ Enterococcus faecium (78%) and VanB+ Enterococcus faecalis (10%). In addition, five VanA+ E. faecium isolates were obtained from environmental and staff sampling. Macrorestriction analysis of SmaI restriction fragment length polymorphism was performed for 54 VanA+ E. faecium clinical isolates and the five VanA+ E. faecium environmental isolates. Thirty-two unique restriction endonuclease patterns were identified, including two predominant clonal types represented by five or more isolates. Two environmental VanA+ E. faecium isolates were closely related to two patient isolates, which had an identical SmaI macrorestriction pattern.
  • This study describes the first molecular characterisation of clinical isolates of vancomycin-resistant enterococci (VRE) in the Czech Republic. Of 2647 patient isolates of Enterococcus spp. from 1997-2002, 121 (4.6%) were identified as VRE. The most common isolates were VanA+ Enterococcus faecium (78%) and VanB+ Enterococcus faecalis (10%). In addition, five VanA+ E. faecium isolates were obtained from environmental and staff sampling. Macrorestriction analysis of SmaI restriction fragment length polymorphism was performed for 54 VanA+ E. faecium clinical isolates and the five VanA+ E. faecium environmental isolates. Thirty-two unique restriction endonuclease patterns were identified, including two predominant clonal types represented by five or more isolates. Two environmental VanA+ E. faecium isolates were closely related to two patient isolates, which had an identical SmaI macrorestriction pattern. (en)
Title
  • Genotypic characterisation of vancomycin-resistant <I>Enterococcus faecium</I> isolates from haemato-oncological patients at Olomouc University Hospital, Czech Republic
  • Genotypic characterisation of vancomycin-resistant <I>Enterococcus faecium</I> isolates from haemato-oncological patients at Olomouc University Hospital, Czech Republic (en)
skos:prefLabel
  • Genotypic characterisation of vancomycin-resistant <I>Enterococcus faecium</I> isolates from haemato-oncological patients at Olomouc University Hospital, Czech Republic
  • Genotypic characterisation of vancomycin-resistant <I>Enterococcus faecium</I> isolates from haemato-oncological patients at Olomouc University Hospital, Czech Republic (en)
skos:notation
  • RIV/00216224:14310/06:00016652!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita
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  • Z(MSM0021622415)
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  • 4
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  • 476921
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  • RIV/00216224:14310/06:00016652
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  • Enterococcus faecium; molecular epidemiology; pulsed-field gel electrophoresis; vancomycin-resistant enterococci (en)
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  • GB - Spojené království Velké Británie a Severního Irska
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  • [F50C8CE44948]
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  • Clinical Microbiology and Infection
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  • 12
http://linked.open...iv/tvurceVysledku
  • Doškař, Jiří
  • Kolář, Milan
  • Pantůček, Roman
  • Koukalová, Dagmar
  • Vágnerová, Iva
  • Sauer, Pavel
  • Hejnar, Petr
  • Kesselová, Michaela
  • Matoušková, Ivana
http://linked.open...ain/vavai/riv/wos
  • 000235661400009
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  • 1198-743X
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