About: Evaluation of amplified ribosomal DNA restriction analysis (ARDRA) and species-specific PCR for identification of Bifidobacterium species

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About: Evaluation of amplified ribosomal DNA restriction analysis (ARDRA) and species-specific PCR for identification of Bifidobacterium species Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Molecular biological methods based on genus and species specific PCR and restriction analysis of two PCR amplicons using six restriction enzymes were used to differentiate among species of Bifidobacterium. Optimalised methods were applied for identification of strains collected in Culture Collection of Dairy Microorganisms. Restriction analysis of longer PCR amplicon was the most discriminative for Bifidobacterium species differentiation. Practicaly observed restriction profiles were compared and confirmed with the theoretical restriction analysis of Bifidobacterium sequences obtained from GenBank database. Molecular biological methods based on genus and species specific PCR and restriction analysis of two PCR amplicons using six restriction enzymes were used to differentiate among species of Bifidobacterium. Optimalised methods were applied for identification of strains collected in Culture Collection of Dairy Microorganisms. Restriction analysis of longer PCR amplicon was the most discriminative for Bifidobacterium species differentiation. Practicaly observed restriction profiles were compared and confirmed with the theoretical restriction analysis of Bifidobacterium sequences obtained from GenBank database. (en)
Title	Evaluation of amplified ribosomal DNA restriction analysis (ARDRA) and species-specific PCR for identification of Bifidobacterium species Evaluation of amplified ribosomal DNA restriction analysis (ARDRA) and species-specific PCR for identification of Bifidobacterium species (en)
skos:prefLabel	Evaluation of amplified ribosomal DNA restriction analysis (ARDRA) and species-specific PCR for identification of Bifidobacterium species Evaluation of amplified ribosomal DNA restriction analysis (ARDRA) and species-specific PCR for identification of Bifidobacterium species (en)
skos:notation	RIV/00216224:14310/06:00016570!RIV10-MZE-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1G46045), P(QF3169), Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Španová, Alena Rittich, Bohuslav Křížová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	474678
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/06:00016570
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Bifidobacterium spp.; identification; PCR; ARDRA (en)
http://linked.open.../riv/klicoveSlovo	PCR identification Bifidobacterium spp. ARDRA
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[BD5ED05C7AB5]
http://linked.open...i/riv/nazevZdroje	Systematic and Applied Microbiology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular biological analysis of inland (Czech) stocks of lactic acid bacteria production strains. The use of procedures on basis of molecular genetics in acquiring of new bacterial strains with probiotic properties with the aim to increase specific genetic sources for production of functional foods.
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	29
http://linked.open...iv/tvurceVysledku	Rittich, Bohuslav Španová, Alena Křížová, Jana
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
issn	0723-2020
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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