About: Isolation of microbial DNA by newly designed magnetic particles

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Carboxyl group-containing magnetic nonporous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres and cobalt ferrite nanoparticles modified with alginic acid (natural carboxylic polysaccharide) were used for isolation of microbial DNA of lactic acid bacteria (LAB) and Trichophyton fungi DNA from lyophilized samples, from a bacterial colony grown on hard media, and from dairy products. DNA from the samples with lysed cells was reversibly adsorbed to the particles in the presence of high poly(ethylene glycol) (PEG 6000) and sodium chloride concentrations. The optimal final PEG and NaCl concentrations were 9.1 wt. % and 2.0 M, respectively. The adsorbed DNA was released from the particles in low ionic strength TE buffer. The quality of isolated DNA was checked by PCR amplification. Moreover, PCR amplicons were isolated on P(HEMA-co-GMA) microspheres and checked by restriction analysis. Carboxyl group-containing magnetic nonporous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres and cobalt ferrite nanoparticles modified with alginic acid (natural carboxylic polysaccharide) were used for isolation of microbial DNA of lactic acid bacteria (LAB) and Trichophyton fungi DNA from lyophilized samples, from a bacterial colony grown on hard media, and from dairy products. DNA from the samples with lysed cells was reversibly adsorbed to the particles in the presence of high poly(ethylene glycol) (PEG 6000) and sodium chloride concentrations. The optimal final PEG and NaCl concentrations were 9.1 wt. % and 2.0 M, respectively. The adsorbed DNA was released from the particles in low ionic strength TE buffer. The quality of isolated DNA was checked by PCR amplification. Moreover, PCR amplicons were isolated on P(HEMA-co-GMA) microspheres and checked by restriction analysis. (en)
Title	Isolation of microbial DNA by newly designed magnetic particles Isolation of microbial DNA by newly designed magnetic particles (en)
skos:prefLabel	Isolation of microbial DNA by newly designed magnetic particles Isolation of microbial DNA by newly designed magnetic particles (en)
skos:notation	RIV/00216224:14310/06:00015958!RIV10-MZE-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1G57037), P(GA203/05/2256), Z(AV0Z40500505), Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Španová, Alena Rittich, Bohuslav Klesnilová, Lenka Petrová, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	480518
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/06:00015958
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Magnetic particles P(HEMA-co-GMA); cobalt ferrite magnetic paricles; microbial DNA; polymerase chain reaction (PCR) (en)
http://linked.open.../riv/klicoveSlovo	Magnetic particles P(HEMA-co-GMA) polymerase chain reaction (PCR) cobalt ferrite magnetic paricles microbial DNA
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[D9E45BED296A]
http://linked.open...i/riv/nazevZdroje	Colloids and Surfaces B: Biointerferences
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Application of molecular biology methods for the effective use of genetic sources of LAB and the characterisation of functional foods quality. Magnetic stimuli-responsive hydrophilic polymer microspheres for biomedicine/bioengineering
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	52
http://linked.open...iv/tvurceVysledku	Horák, Daniel Rittich, Bohuslav Španová, Alena Beneš, Milan Petrová, Kateřina Rybnikář, Alois Klesnilová, Lenka
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie Molecular basis of cell and tissue regulations
issn	0927-7765
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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