About: Proteomic characterization of the polyvalent staphylophage 812

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Bacteriophage 812 is a polyvalent phage with very broad host range in the genus Staphylococcus, which makes it a suitable candidate for phage therapy of staphylococcal infections. The genomes of polyvalent staphylococcal phages exceed 125 kbp and include about 200 putative open reading frames. Our study was focused on characterization of the phage proteome, especially on identification of phage virion proteins. Combination of both one- and two-dimensional electrophoreses (1-DE and 2-DE) followed by peptide mass fingerprinting using MALDI-TOF mass spectrometry, led to the identification of 24 virion proteins of the phage 812. Twenty of them were not identified by proteome analysis of closely related staphylococcal phages K and G1 yet. Although the phage proteome is much simpler compared to that of cellular organisms, using 2-DE in addition to 1-DE significantly increased the number of identified proteins. Bacteriophage 812 is a polyvalent phage with very broad host range in the genus Staphylococcus, which makes it a suitable candidate for phage therapy of staphylococcal infections. The genomes of polyvalent staphylococcal phages exceed 125 kbp and include about 200 putative open reading frames. Our study was focused on characterization of the phage proteome, especially on identification of phage virion proteins. Combination of both one- and two-dimensional electrophoreses (1-DE and 2-DE) followed by peptide mass fingerprinting using MALDI-TOF mass spectrometry, led to the identification of 24 virion proteins of the phage 812. Twenty of them were not identified by proteome analysis of closely related staphylococcal phages K and G1 yet. Although the phage proteome is much simpler compared to that of cellular organisms, using 2-DE in addition to 1-DE significantly increased the number of identified proteins. (en)
Title	Proteomic characterization of the polyvalent staphylophage 812 Proteomic characterization of the polyvalent staphylophage 812 (en)
skos:prefLabel	Proteomic characterization of the polyvalent staphylophage 812 Proteomic characterization of the polyvalent staphylophage 812 (en)
skos:notation	RIV/00216224:14310/06:00015836!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/03/0515), Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Preisler, Jan Hernychová, Lenka Doškař, Jiří Růžičková, Vladislava Zdráhal, Zbyněk Pantůček, Roman Konečná, Hana Eyer, Luděk
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	495704
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/06:00015836
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Staphylococcus aureus; medical microbiology; molecular diagnostics; phage therapy; proteomics (en)
http://linked.open.../riv/klicoveSlovo	medical microbiology molecular diagnostics Staphylococcus aureus proteomics phage therapy
http://linked.open...ontrolniKodProRIV	[A7D85661D03C]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	8 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated genome and proteome analysis of therapeutically important bacteriophages by combination of electrophoresis and mass spectrometry
http://linked.open...UplatneniVysledku	2006
http://linked.open...iv/tvurceVysledku	Doškař, Jiří Hernychová, Lenka Konečná, Hana Pantůček, Roman Preisler, Jan Růžičková, Vladislava Zdráhal, Zbyněk Eyer, Luděk
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
http://localhost/t...ganizacniJednotka	14310

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