About: Determination of cyanide in microliter samples by CE using on-capillary enzymatic reaction with rhodanese

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About: Determination of cyanide in microliter samples by CE using on-capillary enzymatic reaction with rhodanese Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Thirteen years ago a new application for the evaluation of enzymatic reactions in capillary electrophoresis was proposed and developed, electrophoretically mediated microanalysis (EMMA). In this method the capillary is used not only as a separation medium but also as a reaction chamber. Since its discovery by Bao and Regnier [1], the EMMA methodology has been utilized in a number of biochemical systems [2,3]. In this work, the EMMA method was applied to determine cyanide after its on-capillary enzymatic reaction with rhodanese (thiosulfate: cyanide sulfur transferase, EC 2.8.1.1).Consequnetly cyanide thus could be determine as thiocyanate with adequate sensitivity by means of CE with simple UV detection. Compared to other assays, the EMMA method is rapid, can be automated, and requires only small amount of samples. Thirteen years ago a new application for the evaluation of enzymatic reactions in capillary electrophoresis was proposed and developed, electrophoretically mediated microanalysis (EMMA). In this method the capillary is used not only as a separation medium but also as a reaction chamber. Since its discovery by Bao and Regnier [1], the EMMA methodology has been utilized in a number of biochemical systems [2,3]. In this work, the EMMA method was applied to determine cyanide after its on-capillary enzymatic reaction with rhodanese (thiosulfate: cyanide sulfur transferase, EC 2.8.1.1).Consequnetly cyanide thus could be determine as thiocyanate with adequate sensitivity by means of CE with simple UV detection. Compared to other assays, the EMMA method is rapid, can be automated, and requires only small amount of samples. (en)
Title	Determination of cyanide in microliter samples by CE using on-capillary enzymatic reaction with rhodanese Determination of cyanide in microliter samples by CE using on-capillary enzymatic reaction with rhodanese (en)
skos:prefLabel	Determination of cyanide in microliter samples by CE using on-capillary enzymatic reaction with rhodanese Determination of cyanide in microliter samples by CE using on-capillary enzymatic reaction with rhodanese (en)
skos:notation	RIV/00216224:14310/06:00015670!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA525/04/1309), P(LC06023), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Papežová, Kateřina Glatz, Zdeněk
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	471124
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/06:00015670
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	CE; Cyanide; Rhodanese (en)
http://linked.open.../riv/klicoveSlovo	Cyanide Rhodanese CE
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[6941477D489F]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated bioanalytical technologies for microanalyses and diagnostics with laser induced fluorescence and mass spectrometry coupling. Bacterial oxidation of inorganic sulfur substances, environmental aspects
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	1120
http://linked.open...iv/tvurceVysledku	Glatz, Zdeněk Papežová, Kateřina
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
issn	0021-9673
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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