About: Ergosterol treatment leads to the expression of a specific set of defence-related genes in tobacco     Goto   Sponge   NotDistinct   Permalink

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  • Ergosterol is the main sterol of most fungi. Production of reactive oxygen species after the treatment of tobacco and tomato cells by nano-molar concentrations of ergosterol was previously observed as well as the activation of some stress activated mitogen-activated protein kinases on alfalfa cells. In this paper, the expression of some defence-related genes after the ergosterol treatment of tobacco Nicotiana tabacum plants is reported. The gene expression of pathogenesis related proteins of families PR1, PR3, PR5 and proteinase inhibitors of class I and II together with enzymes participating in the defence response, such as phenylalanine ammonia lyase and sesquiterpene cyclase, were monitored by RT-qPCR. In addition, the concentrations of salicylic acid, an important signalling molecule, increased in time due to the enzyme activation. On the other hand, ergosterol did not provoke tissue necrosis and the possible cross-talk between the signalling pathways of salicylate and jasmonate was observed.
  • Ergosterol is the main sterol of most fungi. Production of reactive oxygen species after the treatment of tobacco and tomato cells by nano-molar concentrations of ergosterol was previously observed as well as the activation of some stress activated mitogen-activated protein kinases on alfalfa cells. In this paper, the expression of some defence-related genes after the ergosterol treatment of tobacco Nicotiana tabacum plants is reported. The gene expression of pathogenesis related proteins of families PR1, PR3, PR5 and proteinase inhibitors of class I and II together with enzymes participating in the defence response, such as phenylalanine ammonia lyase and sesquiterpene cyclase, were monitored by RT-qPCR. In addition, the concentrations of salicylic acid, an important signalling molecule, increased in time due to the enzyme activation. On the other hand, ergosterol did not provoke tissue necrosis and the possible cross-talk between the signalling pathways of salicylate and jasmonate was observed. (en)
Title
  • Ergosterol treatment leads to the expression of a specific set of defence-related genes in tobacco
  • Ergosterol treatment leads to the expression of a specific set of defence-related genes in tobacco (en)
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  • Ergosterol treatment leads to the expression of a specific set of defence-related genes in tobacco
  • Ergosterol treatment leads to the expression of a specific set of defence-related genes in tobacco (en)
skos:notation
  • RIV/00216224:14310/06:00015653!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA522/06/0156), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika
  • 1-2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
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http://linked.open...dnocenehoVysledku
  • 474403
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/06:00015653
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • defence genes; Nicotiana tabacum; ergosterol; elicitor; salicylic acid; plant defence (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [E59D2C3EE6D7]
http://linked.open...i/riv/nazevZdroje
  • Plant Molecular Biology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 62
http://linked.open...iv/tvurceVysledku
  • Lochman, Jan
  • Mikeš, Vladimír
http://linked.open...n/vavai/riv/zamer
issn
  • 0167-4412
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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