About: High-Throughput Axial MALDI-TOF MS Using a 2 kHz Repetition Rate Laser

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A high-throughput axial MALDI-TOF mass spectrometer utilizing a laser with a 2 kHz pulse repetition-rate was constructed and tested. This fast mass spectrometer provided a data acquisition rate 10 times faster than a commercially available (200 Hz) axial mass spectrometer, while maintaining comparable limits of detection (200 amole of Glu fib peptide). Mass resolution, only slightly less than the commercial instrument (10,000 vs. 14,000), was sufficient for baseline resolution of isotopic clusters of peptides with m/z <2700. A new method of mass calibration, which combined a limited number of internal and external standards, provided the same 15 ppm mass accuracy over the entire sample plate on either instrument. Implementing the 2 kHz laser required a faster data acquisition system and high-voltage pulse electronics, together with a novel strategy for rapid sample plate movements during acquisition, to achieve a sample analysis rate of up to 2 spots per second (with 800 shots/spot). A high-throughput axial MALDI-TOF mass spectrometer utilizing a laser with a 2 kHz pulse repetition-rate was constructed and tested. This fast mass spectrometer provided a data acquisition rate 10 times faster than a commercially available (200 Hz) axial mass spectrometer, while maintaining comparable limits of detection (200 amole of Glu fib peptide). Mass resolution, only slightly less than the commercial instrument (10,000 vs. 14,000), was sufficient for baseline resolution of isotopic clusters of peptides with m/z <2700. A new method of mass calibration, which combined a limited number of internal and external standards, provided the same 15 ppm mass accuracy over the entire sample plate on either instrument. Implementing the 2 kHz laser required a faster data acquisition system and high-voltage pulse electronics, together with a novel strategy for rapid sample plate movements during acquisition, to achieve a sample analysis rate of up to 2 spots per second (with 800 shots/spot). (en)
Title	High-Throughput Axial MALDI-TOF MS Using a 2 kHz Repetition Rate Laser High-Throughput Axial MALDI-TOF MS Using a 2 kHz Repetition Rate Laser (en)
skos:prefLabel	High-Throughput Axial MALDI-TOF MS Using a 2 kHz Repetition Rate Laser High-Throughput Axial MALDI-TOF MS Using a 2 kHz Repetition Rate Laser (en)
skos:notation	RIV/00216224:14310/06:00015575!RIV10-GA0-14310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA203/03/0515)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Preisler, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	477733
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/06:00015575
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	MALDI; TOF; mass spectrometry; time of flight; high-throughput (en)
http://linked.open.../riv/klicoveSlovo	mass spectrometry TOF MALDI high-throughput time of flight
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[CC65D6414449]
http://linked.open...i/riv/nazevZdroje	Analytical Chemistry
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated genome and proteome analysis of therapeutically important bacteriophages by combination of electrophoresis and mass spectrometry
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	78
http://linked.open...iv/tvurceVysledku	Preisler, Jan Moskovets, Eugene Chen, H. S. Rejtar, Tomáš Karger, Barry L. Andreev, Viktor
http://linked.open...ain/vavai/riv/wos	000235195200050
issn	0003-2700
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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