About: Sensitive Detection and Separation of Fluorescent Derivatives using Capillary Electrophoresis with Laser-Induced Fluorescence Detection with 532 nm Nd:YAG Laser

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About: Sensitive Detection and Separation of Fluorescent Derivatives using Capillary Electrophoresis with Laser-Induced Fluorescence Detection with 532 nm Nd:YAG Laser Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Capillary Electrophoresis with Laser Induced Fluorescence detection (CELIF) is a powerful tool for separation and sensitive determination of fluorescent compounds. Biologically active compounds, such as amino acids, peptides and proteins may exhibit native fluorescence, which is however often low and/or an expensive laser is required for excitation in UV. Therefore, labeling of the analytes with a fluorescent dye is usually necessary. In this work, home-built CELIF instrument was developed and tuned up using rhodamine-based dyes with diode pumped frequency-doubled continuous wave Nd:YAG excitation laser with feedback power regulation (532 nm). First, the suitability and performance of this type of laser for LIF detection in a separation method was evaluated. Detection limit of 2E-13 M was achieved with rhodamine B, which is comparable to previously described CELIF with Ar+ laser. An exemplary separation of rhodamine dyes is given. Capillary Electrophoresis with Laser Induced Fluorescence detection (CELIF) is a powerful tool for separation and sensitive determination of fluorescent compounds. Biologically active compounds, such as amino acids, peptides and proteins may exhibit native fluorescence, which is however often low and/or an expensive laser is required for excitation in UV. Therefore, labeling of the analytes with a fluorescent dye is usually necessary. In this work, home-built CELIF instrument was developed and tuned up using rhodamine-based dyes with diode pumped frequency-doubled continuous wave Nd:YAG excitation laser with feedback power regulation (532 nm). First, the suitability and performance of this type of laser for LIF detection in a separation method was evaluated. Detection limit of 2E-13 M was achieved with rhodamine B, which is comparable to previously described CELIF with Ar+ laser. An exemplary separation of rhodamine dyes is given. (en)
Title	Sensitive Detection and Separation of Fluorescent Derivatives using Capillary Electrophoresis with Laser-Induced Fluorescence Detection with 532 nm Nd:YAG Laser Sensitive Detection and Separation of Fluorescent Derivatives using Capillary Electrophoresis with Laser-Induced Fluorescence Detection with 532 nm Nd:YAG Laser (en)
skos:prefLabel	Sensitive Detection and Separation of Fluorescent Derivatives using Capillary Electrophoresis with Laser-Induced Fluorescence Detection with 532 nm Nd:YAG Laser Sensitive Detection and Separation of Fluorescent Derivatives using Capillary Electrophoresis with Laser-Induced Fluorescence Detection with 532 nm Nd:YAG Laser (en)
skos:notation	RIV/00216224:14310/06:00015565!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/03/0515), Z(MSM 143100011)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Ryvolová, Markéta Havel, Josef Preisler, Jan Táborský, Petr Vrábel, Patrik
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	498886
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/06:00015565
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Electrophoresis; Fluorescence; Derivatization; RBITC; Proteins; Rhodamine; 532 nm Laser. (en)
http://linked.open.../riv/klicoveSlovo	Proteins Derivatization Fluorescence Electrophoresis 532 nm Laser. RBITC Rhodamine
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[A497B73DB8DB]
http://linked.open...i/riv/nazevZdroje	Journal of Luminescence
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated genome and proteome analysis of therapeutically important bacteriophages by combination of electrophoresis and mass spectrometry
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	118
http://linked.open...iv/tvurceVysledku	Havel, Josef Preisler, Jan Ryvolová, Markéta Táborský, Petr Vrábel, Patrik
http://linked.open...ain/vavai/riv/wos	000235852800021
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20143100011
issn	0022-2313
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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