About: pOp6/LhGR: a stringently regulated and highly responsive dexamethasone-inducible gene expression system for tobacco.

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About: pOp6/LhGR: a stringently regulated and highly responsive dexamethasone-inducible gene expression system for tobacco. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	pOp/LhGR, a dexamethasone-inducible derivative of the pOp/LhG4 transcription activation system, and its use in tobacco to regulate expression of uidA (encoding beta-glucuronidase; GUS) and the cytokinin-biosynthetic gene ipt is described. The pOp/LhGR system exhibited stringent regulation and strong induced phenotypes in soil and tissue culture. A single dose of dexamethasone was sufficient to direct cytotoxic levels of ipt expression in soil grown plants although uninduced plants grew normally throughout a complete life cycle. In vitro, induced transcripts were detectable within an hour of dexamethasone application and 1nM dexamethasone was sufficient for half maximal induction of GUS activity. Various methods of dexamethasone application were successfully applied under tissue culture and greenhouse conditions. No inhibitory effects of dexamethasone or LhGR on plant development even with the highest concentrations of inducer were observed. pOp/LhGR, a dexamethasone-inducible derivative of the pOp/LhG4 transcription activation system, and its use in tobacco to regulate expression of uidA (encoding beta-glucuronidase; GUS) and the cytokinin-biosynthetic gene ipt is described. The pOp/LhGR system exhibited stringent regulation and strong induced phenotypes in soil and tissue culture. A single dose of dexamethasone was sufficient to direct cytotoxic levels of ipt expression in soil grown plants although uninduced plants grew normally throughout a complete life cycle. In vitro, induced transcripts were detectable within an hour of dexamethasone application and 1nM dexamethasone was sufficient for half maximal induction of GUS activity. Various methods of dexamethasone application were successfully applied under tissue culture and greenhouse conditions. No inhibitory effects of dexamethasone or LhGR on plant development even with the highest concentrations of inducer were observed. (en)
Title	pOp6/LhGR: a stringently regulated and highly responsive dexamethasone-inducible gene expression system for tobacco. pOp6/LhGR: a stringently regulated and highly responsive dexamethasone-inducible gene expression system for tobacco. (en)
skos:prefLabel	pOp6/LhGR: a stringently regulated and highly responsive dexamethasone-inducible gene expression system for tobacco. pOp6/LhGR: a stringently regulated and highly responsive dexamethasone-inducible gene expression system for tobacco. (en)
skos:notation	RIV/00216224:14310/05:00019362!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA5004001), P(KSK5052113), Z(AV0Z50040507), Z(MSM 143100008)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Brzobohatý, Břetislav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	536761
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/05:00019362
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	chemically inducible gene expression; cytokinin-biosynthetic gene ipt; tobacco (en)
http://linked.open.../riv/klicoveSlovo	chemically inducible gene expression cytokinin-biosynthetic gene ipt tobacco
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[9422B7A699A3]
http://linked.open...i/riv/nazevZdroje	The Plant Journal
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Structure, expression and interaction of the genome Transkription activation system for studying the relationship between cytokinin metabolism and action in Arabidopsis and tobacco
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	41
http://linked.open...iv/tvurceVysledku	Brzobohatý, Břetislav Šámalová, Markéta Moore, Ian
http://linked.open...n/vavai/riv/zamer	Biophysics of dynamic structures and functions of biological systems http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20143100008
issn	0960-7412
number of pages	17 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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