About: Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase     Goto   Sponge   NotDistinct   Permalink

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Description
  • A screen-printed four-electrode sensor based on immobilization of laccase (Coriolus hirsutus), peroxidase (horseradish) and tyrosinase (mushroom) in the same array was developed for monitoring of phenols. The enzymes were immobilized onto a self-assembled monolayer (4-mercapto-1-butanol) modified gold surface via covalent attachment by epichlorohydrin coupling. The experimental conditions for simultaneous operation of the three enzymes were optimized based on catechol determination. The sensors were further applied for the amperometric detection of several substituted phenolic compounds, carried out using a single line flow-injection system. Hydrogen peroxide served as co-substrate for peroxidase. The limits of detection for phenols in aqueous solutions were in the micromolar range, one assay was completed in less than 5 min.
  • A screen-printed four-electrode sensor based on immobilization of laccase (Coriolus hirsutus), peroxidase (horseradish) and tyrosinase (mushroom) in the same array was developed for monitoring of phenols. The enzymes were immobilized onto a self-assembled monolayer (4-mercapto-1-butanol) modified gold surface via covalent attachment by epichlorohydrin coupling. The experimental conditions for simultaneous operation of the three enzymes were optimized based on catechol determination. The sensors were further applied for the amperometric detection of several substituted phenolic compounds, carried out using a single line flow-injection system. Hydrogen peroxide served as co-substrate for peroxidase. The limits of detection for phenols in aqueous solutions were in the micromolar range, one assay was completed in less than 5 min. (en)
Title
  • Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase
  • Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase (en)
skos:prefLabel
  • Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase
  • Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase (en)
skos:notation
  • RIV/00216224:14310/05:00014346!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita
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  • Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika
  • 23
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 511861
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/05:00014346
http://linked.open...riv/jazykVysledku
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  • electrochemical biosensor; enzyme electrode; phenol; catechol; waste water (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [48C1A83542AB]
http://linked.open...i/riv/nazevZdroje
  • Electroanalysis
http://linked.open...in/vavai/riv/obor
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 17
http://linked.open...iv/tvurceVysledku
  • Skládal, Petr
  • Solná, Renáta
http://linked.open...n/vavai/riv/zamer
issn
  • 1040-0397
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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