About: Molecular analysis of FRI (FRIGIDA) gene in early and late-flowering ecotypes and late-flowering mutants in Arabidopsis thaliana.     Goto   Sponge   NotDistinct   Permalink

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  • Flowering time is stimulated by both environmental (light, temperature) and endogenous signals. FLC and FRI genes are the most important endogenous signals controlling onset of flowering in natural populations. FLC is a significant flowering repressor that is under the control of the FRI gene. Interaction between of both genes is reason of lateness in natural populations. In the FRI gene two frequent deletions are known, Ler- and Col-type deletions, which are responsible for early-flowering phenotype. These deletions have been identified only in early-flowering plants but never in late-flowering therefore we focused on molecular analysis of FRI gene in late-flowering ecotypes from the Czech Republic (Je-4, Je-18, Je-27, Je-28 and Hod) and in four late-flowering mutants (dn, L4, Spi and M73). For this purpose we used five PCR primers that cover the complete sequence of FRI gene including the promoter. Deletions or insertions were detected by agarose or polyacrylamide gels.
  • Flowering time is stimulated by both environmental (light, temperature) and endogenous signals. FLC and FRI genes are the most important endogenous signals controlling onset of flowering in natural populations. FLC is a significant flowering repressor that is under the control of the FRI gene. Interaction between of both genes is reason of lateness in natural populations. In the FRI gene two frequent deletions are known, Ler- and Col-type deletions, which are responsible for early-flowering phenotype. These deletions have been identified only in early-flowering plants but never in late-flowering therefore we focused on molecular analysis of FRI gene in late-flowering ecotypes from the Czech Republic (Je-4, Je-18, Je-27, Je-28 and Hod) and in four late-flowering mutants (dn, L4, Spi and M73). For this purpose we used five PCR primers that cover the complete sequence of FRI gene including the promoter. Deletions or insertions were detected by agarose or polyacrylamide gels. (en)
Title
  • Molecular analysis of FRI (FRIGIDA) gene in early and late-flowering ecotypes and late-flowering mutants in Arabidopsis thaliana.
  • Molecular analysis of FRI (FRIGIDA) gene in early and late-flowering ecotypes and late-flowering mutants in Arabidopsis thaliana. (en)
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  • Molecular analysis of FRI (FRIGIDA) gene in early and late-flowering ecotypes and late-flowering mutants in Arabidopsis thaliana.
  • Molecular analysis of FRI (FRIGIDA) gene in early and late-flowering ecotypes and late-flowering mutants in Arabidopsis thaliana. (en)
skos:notation
  • RIV/00216224:14310/05:00014020!RIV10-MSM-14310___
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  • RIV/00216224:14310/05:00014020
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  • Arabidopsis; late-flowering; FRI (en)
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  • [D1F26BB5FEF1]
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  • Řepková, Jana
  • Cetkovská, Kateřina
  • Lízal, Pavel
  • Relichová, Jiřina
  • Nešpor Dadejová, Martina
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  • Ústav molekulární biologie rostlin AV ČR
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  • 80-86778-16-9
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  • 14310
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