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  • A set of reference strains and a group of previously unidentified enterococci were analysed by rep-PCR with the (GTG)5 primer to evaluate the discriminatory power and suitability of this method for typing and identification of enterococcal species. A total of 49 strains representing all validly described species were obtained from bacterial collections. For more extensive evaluation of this identification approach 112 well-defined and identified enterococci isolated from bryndza cheese were tested. The (GTG)5-PCR fingerprinting assigned all strains into well-differentiated clusters representing individual species. Subsequently, a group including 44 unidentified enterococci isolated from surface waters was analysed to evaluate this method for identification of unknown isolates. Obtained band patterns allowed us to identify all the strains clearly to the species level. This study proved that rep-PCR with (GTG)5 primer is a reliable and fast method for species identification of enterococci.
  • A set of reference strains and a group of previously unidentified enterococci were analysed by rep-PCR with the (GTG)5 primer to evaluate the discriminatory power and suitability of this method for typing and identification of enterococcal species. A total of 49 strains representing all validly described species were obtained from bacterial collections. For more extensive evaluation of this identification approach 112 well-defined and identified enterococci isolated from bryndza cheese were tested. The (GTG)5-PCR fingerprinting assigned all strains into well-differentiated clusters representing individual species. Subsequently, a group including 44 unidentified enterococci isolated from surface waters was analysed to evaluate this method for identification of unknown isolates. Obtained band patterns allowed us to identify all the strains clearly to the species level. This study proved that rep-PCR with (GTG)5 primer is a reliable and fast method for species identification of enterococci. (en)
Title
  • Evaluation of (GTG)5-PCR for identification of Enterococcus spp.
  • Evaluation of (GTG)5-PCR for identification of Enterococcus spp. (en)
skos:prefLabel
  • Evaluation of (GTG)5-PCR for identification of Enterococcus spp.
  • Evaluation of (GTG)5-PCR for identification of Enterococcus spp. (en)
skos:notation
  • RIV/00216224:14310/05:00013780!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM0021622416)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 520724
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/05:00013780
http://linked.open...riv/jazykVysledku
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  • Enterococcus; rep-PCR; (GTG)5-PCR; identification; bryndza; water (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [A16B43309209]
http://linked.open...i/riv/nazevZdroje
  • FEMS Microbiology Letters
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 247
http://linked.open...iv/tvurceVysledku
  • Sedláček, Ivo
  • Švec, Pavel
  • Seman, Milan
  • Swings, Jean
  • Vancanneyt, Marc
  • Lefebvre, Karen
  • Snauwaert, Cindy
http://linked.open...ain/vavai/riv/wos
  • 000229707200008
http://linked.open...n/vavai/riv/zamer
issn
  • 0378-1097
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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