About: Quartz crystal biosensor for detection of sugars and glycated hemoglobin

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	3-Aminophenylboronic acid (APBA) was used for construction of affinity mass sensors for determination of saccharides and glycated hemoglobin using complexation reaction with diol groups. Two approaches were tested for the bioligand layer fabrication-incorporation of APBA inside a thicker matrix and immobilization as a self-assembled monolayer, respectively. The direct affinity sensor with APBA embeded in the structure of glutaraldehyde-crosslinked bovine serum albumine provided linear response to mono- and dissacharides in the range from 0.1 to 15.0 mg/ml. This biosensor was also used for kinetic analysis of the interaction of boronic acid with diols; the values of association and dissociation constants were determined. The sensors with a monolayer of boronic groups were better suited for binding of glycated hemoglobin, probably due to improved steric access to the ligand. 3-Aminophenylboronic acid (APBA) was used for construction of affinity mass sensors for determination of saccharides and glycated hemoglobin using complexation reaction with diol groups. Two approaches were tested for the bioligand layer fabrication-incorporation of APBA inside a thicker matrix and immobilization as a self-assembled monolayer, respectively. The direct affinity sensor with APBA embeded in the structure of glutaraldehyde-crosslinked bovine serum albumine provided linear response to mono- and dissacharides in the range from 0.1 to 15.0 mg/ml. This biosensor was also used for kinetic analysis of the interaction of boronic acid with diols; the values of association and dissociation constants were determined. The sensors with a monolayer of boronic groups were better suited for binding of glycated hemoglobin, probably due to improved steric access to the ligand. (en)
Title	Quartz crystal biosensor for detection of sugars and glycated hemoglobin Quartz crystal biosensor for detection of sugars and glycated hemoglobin (en)
skos:prefLabel	Quartz crystal biosensor for detection of sugars and glycated hemoglobin Quartz crystal biosensor for detection of sugars and glycated hemoglobin (en)
skos:notation	RIV/00216224:14310/05:00013672!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Skládal, Petr Přibyl, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	539865
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/05:00013672
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	quartz crystal microbalance; m-aminophenylboronic acid; saccharides; glycated hemoglobin; kinetic analysis (en)
http://linked.open.../riv/klicoveSlovo	glycated hemoglobin saccharides m-aminophenylboronic acid kinetic analysis quartz crystal microbalance
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[1773141FF5CB]
http://linked.open...i/riv/nazevZdroje	Analytica Chimica Acta
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	530
http://linked.open...iv/tvurceVysledku	Skládal, Petr Přibyl, Jan
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
issn	0003-2670
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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