About: The trnL-F plastid DNA characters of three Poa pratensis (Kentucky bluegrass) varieties     Goto   Sponge   NotDistinct   Permalink

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  • The characterization of crop cultivars (varieties) will come to depend increasingly on molecular characters in addition to traditional morphological and agronomic characters. Three cultivars of Kentucky bluegrass (Poa pratensis L.), developed by the Plant Breeding Station Hladké Životice (PBHŽ), were characterized using sequences and PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) patterns from the non-coding plastid trnL-F region (trnL intron, 549 bp, and trnL-trnF intergenic spacer [IGS], 344 to 364 bp). These characters could be readily and repeatably determined not only for mature plants, but also for seedlings (less than 12 weeks old), which are difficult to distinguish morphologically. The method is quick and sensitive. When restricted with a combination of BsaJ I and Bsm I, cultivar Slezanka has one major band, Moravanka has two, and Harmonie has three. When restricted with Alu I, the heaviest band migrates most slowly for Slezanka.
  • The characterization of crop cultivars (varieties) will come to depend increasingly on molecular characters in addition to traditional morphological and agronomic characters. Three cultivars of Kentucky bluegrass (Poa pratensis L.), developed by the Plant Breeding Station Hladké Životice (PBHŽ), were characterized using sequences and PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) patterns from the non-coding plastid trnL-F region (trnL intron, 549 bp, and trnL-trnF intergenic spacer [IGS], 344 to 364 bp). These characters could be readily and repeatably determined not only for mature plants, but also for seedlings (less than 12 weeks old), which are difficult to distinguish morphologically. The method is quick and sensitive. When restricted with a combination of BsaJ I and Bsm I, cultivar Slezanka has one major band, Moravanka has two, and Harmonie has three. When restricted with Alu I, the heaviest band migrates most slowly for Slezanka. (en)
Title
  • The trnL-F plastid DNA characters of three Poa pratensis (Kentucky bluegrass) varieties
  • The trnL-F plastid DNA characters of three Poa pratensis (Kentucky bluegrass) varieties (en)
skos:prefLabel
  • The trnL-F plastid DNA characters of three Poa pratensis (Kentucky bluegrass) varieties
  • The trnL-F plastid DNA characters of three Poa pratensis (Kentucky bluegrass) varieties (en)
skos:notation
  • RIV/00216224:14310/05:00013561!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM 143100010), Z(MSM0021622416)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 547342
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/05:00013561
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  • cultivar characterization; Harmonie; Moravanka; non-coding plastid DNA sequence; Slezanka; trnL intron; trnL-trnF intergenic spacer; turf grass (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [E18BB293DB08]
http://linked.open...i/riv/nazevZdroje
  • Plant, Soil and Environment
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 51
http://linked.open...iv/tvurceVysledku
  • Bureš, Petr
  • Horová, Lucie
  • Černoch, Vladimír
  • Stoneberg Holt, Sierra Dawn
  • Janeček, Josef
http://linked.open...n/vavai/riv/zamer
issn
  • 1214-1178
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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