About: Study of Fluorescently Labelled Peptides and Small Proteins Using Capillary Electrophoresis Combined with Laser Induced Fluorescence and MALDI MS Detection

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this work, we apply CE-LIF, CE-UV and CE - Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI TOFMS) to investigate derivatization of model peptides and small proteins with rhodamine-based fluorescent reagents, which are compatible with the wavelength of frequency doubled diode pumped Nd:YAG excitation laser (532 nm). While the LIF detection mode is needed for sensitive detection, the MALDI TOFMS analysis can reveal the mass of analytes. Both derivatized and non-derivatized peptides and proteins, the number of fluorescent labels per peptide/proteins and other product of derivatization process can be detected directly from reaction mixtures or after separation. Thus, MALDI TOFMS serves for additional identification of peaks present in CELIF or CEUV electropherograms. For off-line coupling of CE with MALDI MS, subatmospheric deposition interface was used. An examples of final sensitive CELIF separation of derivatized peptides and small proteins are given. In this work, we apply CE-LIF, CE-UV and CE - Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI TOFMS) to investigate derivatization of model peptides and small proteins with rhodamine-based fluorescent reagents, which are compatible with the wavelength of frequency doubled diode pumped Nd:YAG excitation laser (532 nm). While the LIF detection mode is needed for sensitive detection, the MALDI TOFMS analysis can reveal the mass of analytes. Both derivatized and non-derivatized peptides and proteins, the number of fluorescent labels per peptide/proteins and other product of derivatization process can be detected directly from reaction mixtures or after separation. Thus, MALDI TOFMS serves for additional identification of peaks present in CELIF or CEUV electropherograms. For off-line coupling of CE with MALDI MS, subatmospheric deposition interface was used. An examples of final sensitive CELIF separation of derivatized peptides and small proteins are given. (en)
Title	Study of Fluorescently Labelled Peptides and Small Proteins Using Capillary Electrophoresis Combined with Laser Induced Fluorescence and MALDI MS Detection Study of Fluorescently Labelled Peptides and Small Proteins Using Capillary Electrophoresis Combined with Laser Induced Fluorescence and MALDI MS Detection (en)
skos:prefLabel	Study of Fluorescently Labelled Peptides and Small Proteins Using Capillary Electrophoresis Combined with Laser Induced Fluorescence and MALDI MS Detection Study of Fluorescently Labelled Peptides and Small Proteins Using Capillary Electrophoresis Combined with Laser Induced Fluorescence and MALDI MS Detection (en)
skos:notation	RIV/00216224:14310/05:00012704!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/03/0515), Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Ryvolová, Markéta Havel, Josef Preisler, Jan Táborský, Petr Řehulková, Helena Vrábel, Patrik
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	545226
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/05:00012704
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Derivatization; Fluorescent labelling; Capillary Electrophoresis; MALDI Mass Spectrometry; Peptides and Proteins (en)
http://linked.open.../riv/klicoveSlovo	Derivatization Fluorescent labelling MALDI Mass Spectrometry Peptides and Proteins Capillary Electrophoresis
http://linked.open...ontrolniKodProRIV	[D3EE0BC0A509]
http://linked.open...v/mistoKonaniAkce	Praha, ÚOCHB AVČR
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	Collection Symposium Series, Biollogicaly Active Peptides
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated genome and proteome analysis of therapeutically important bacteriophages by combination of electrophoresis and mass spectrometry
http://linked.open...UplatneniVysledku	2005
http://linked.open...iv/tvurceVysledku	Havel, Josef Preisler, Jan Ryvolová, Markéta Táborský, Petr Řehulková, Helena Vrábel, Patrik
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2005-04-20 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
number of pages	4 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Ústav organické chemie a biochemie AV ČR
https://schema.org/isbn	80-86241-26-2
http://localhost/t...ganizacniJednotka	14310

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