About: Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods

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About: Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A new method has been developed for the monitoring of glutathione S-tranferase (GST) detoxification activity toward styrene oxide (SO). The enzymatic reaction was carried out directly in thermostatted autosampler vial and the formation of conjugates between glutathione (GSH) and SO was monitored by sequential MEKC runs. The determinations were performed in a 50 um fused silica capillary using 50 mM SDS in 20 mM phosphate 20 mM tetraborate buffer (pH 8.3) as a background electrolyte; separation voltage 28 kV (positive polarity), temperature of capillary 25 oC and detection at 200 nm. The method is rapid, able to be automated, and requires only small amount of samples, which is especially important in the case of GST isoenzyme analyses. A new method has been developed for the monitoring of glutathione S-tranferase (GST) detoxification activity toward styrene oxide (SO). The enzymatic reaction was carried out directly in thermostatted autosampler vial and the formation of conjugates between glutathione (GSH) and SO was monitored by sequential MEKC runs. The determinations were performed in a 50 um fused silica capillary using 50 mM SDS in 20 mM phosphate 20 mM tetraborate buffer (pH 8.3) as a background electrolyte; separation voltage 28 kV (positive polarity), temperature of capillary 25 oC and detection at 200 nm. The method is rapid, able to be automated, and requires only small amount of samples, which is especially important in the case of GST isoenzyme analyses. (en)
Title	Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods (en)
skos:prefLabel	Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods (en)
skos:notation	RIV/00216224:14310/05:00012561!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/03/1125), P(IBS4031201), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika	12
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Šišková, Zdeňka Glatz, Zdeněk Kahle, Vladislav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	538131
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/05:00012561
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	styrene oxide; GST; MEKC; CEC (en)
http://linked.open.../riv/klicoveSlovo	MEKC CEC styrene oxide GST
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[CD22ACA055E7]
http://linked.open...i/riv/nazevZdroje	Journal of Separation Science
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Application of capillary zone electrophoresis for the study of enzymes Apparatus for automation of electrochromatographic and electrophoretic analysis
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	28
http://linked.open...iv/tvurceVysledku	Kahle, Vladislav Glatz, Zdeněk Šišková, Zdeňka
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
issn	1615-9306
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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