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Description
  • The screen-printed four-electrode system was used as the amperometric transducer for determination of phenols and pesticides using immobilised tyrosinase, peroxidase, acetylcholinesterase and butyrylcholinesterase. Acetylthiocholine chloride was chosen as substrate for cholinesterases to measure inhibition by pesticides, hydrogen peroxide served as co-substrate for peroxidase to measure phenols. The compatibility of hydrolases and oxidoreductases working in the same array was studied. The detection of p-cresol, catechol and phenol as well as of pesticides including carbaryl, heptenophos and fenitrothion was carried out in flow-through and steady state arrangements. In addition, the effects of heavy metals (Cu2+, Cd2+, Fe3+), fluoride (NaF), benzene and dimethylsulphoxide on cholinesterase activities were evaluated. It was demonstrated that electrodes modified with hydrolases and oxidoreductases can function in the same array. The achieved R.S.D.
  • The screen-printed four-electrode system was used as the amperometric transducer for determination of phenols and pesticides using immobilised tyrosinase, peroxidase, acetylcholinesterase and butyrylcholinesterase. Acetylthiocholine chloride was chosen as substrate for cholinesterases to measure inhibition by pesticides, hydrogen peroxide served as co-substrate for peroxidase to measure phenols. The compatibility of hydrolases and oxidoreductases working in the same array was studied. The detection of p-cresol, catechol and phenol as well as of pesticides including carbaryl, heptenophos and fenitrothion was carried out in flow-through and steady state arrangements. In addition, the effects of heavy metals (Cu2+, Cd2+, Fe3+), fluoride (NaF), benzene and dimethylsulphoxide on cholinesterase activities were evaluated. It was demonstrated that electrodes modified with hydrolases and oxidoreductases can function in the same array. The achieved R.S.D. (en)
Title
  • Multienzyme electrochemical array sensor for determination of phenols and pesticides
  • Multienzyme electrochemical array sensor for determination of phenols and pesticides (en)
skos:prefLabel
  • Multienzyme electrochemical array sensor for determination of phenols and pesticides
  • Multienzyme electrochemical array sensor for determination of phenols and pesticides (en)
skos:notation
  • RIV/00216224:14310/05:00012356!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA525/97/0924), Z(MSM0021622413)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 531597
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/05:00012356
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • amperometric screen-printed biosensor; flow-injection analysis; steady state system; tyrosinase; peroxidase; cholinesterase; phenols; pesticides; wastewater (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [1D1C4377D5F4]
http://linked.open...i/riv/nazevZdroje
  • Talanta
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 65
http://linked.open...iv/tvurceVysledku
  • Skládal, Petr
  • Solná, Renáta
  • Emneus, Jenny
  • Ruzgas, Tautgirdas
  • Sapelnikova, Svetlana
  • Carlsson, Charlotte
  • Winther-Nielsen, Margarethe
http://linked.open...n/vavai/riv/zamer
issn
  • 0039-9140
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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