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| rdf:type
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| Description
| - Rostlinný hormon cytokinin hraje klíčovou úlohu ve vývoji rostlin od klíčení po senescenci, nicméně přesné mechanismy jeho vlivu na tyto vývojové procesy nejsou dosud dostatečně objasněny. Naše studie se pokouší přinést více informací o působení cytokininu u modelové rostliny Arabidopsis thaliana. K tomuto účelu jsme využili klasický proteomický přístup založený na separaci proteinů dvourozměrnou elektroforézou následovanou jejich digescí a identifikací pomocí hmotnostní spektrometrie. (cs)
- The plant hormone cytokinin plays a crucial role in plant development from germination to senescence. Nevertheless, the exact mechanisms of its influence on these several developmental processes remain unexplained. We report an attempt to reveal more information about the mode of action of cytokinin in the model plant Arabidopsis thaliana. For this purpose a classical proteome-based approach using two-dimensional electrophoretic separation followed by protein digestion and identification by mass spectrometry was established. Total proteins were extracted from whole Arabidopsis plantlets of following genotypes: wild-type ColO, amp1 mutant accumulating six-fold higher level of endogenous cytokinin (1) and pOp-ipt::LhGR transgenic line in which the production of endogenous cytokinin is inducible by dexamethasone (2). All plants were grown for 15 days under long-day conditions (16h light/8h dark) on MS media, in parallel with and without 2.5 mM dexamethasone. Image analysis of two-dimensional gels in trip
- The plant hormone cytokinin plays a crucial role in plant development from germination to senescence. Nevertheless, the exact mechanisms of its influence on these several developmental processes remain unexplained. We report an attempt to reveal more information about the mode of action of cytokinin in the model plant Arabidopsis thaliana. For this purpose a classical proteome-based approach using two-dimensional electrophoretic separation followed by protein digestion and identification by mass spectrometry was established. Total proteins were extracted from whole Arabidopsis plantlets of following genotypes: wild-type ColO, amp1 mutant accumulating six-fold higher level of endogenous cytokinin (1) and pOp-ipt::LhGR transgenic line in which the production of endogenous cytokinin is inducible by dexamethasone (2). All plants were grown for 15 days under long-day conditions (16h light/8h dark) on MS media, in parallel with and without 2.5 mM dexamethasone. Image analysis of two-dimensional gels in trip (en)
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| Title
| - Effect of endogenous cytokinin levels on the Arabidopsis thaliana proteome
- Effect of endogenous cytokinin levels on the Arabidopsis thaliana proteome (en)
- Vliv hladiny endogenních cytokininů na proteom Arabidopsis thaliana (cs)
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| skos:prefLabel
| - Effect of endogenous cytokinin levels on the Arabidopsis thaliana proteome
- Effect of endogenous cytokinin levels on the Arabidopsis thaliana proteome (en)
- Vliv hladiny endogenních cytokininů na proteom Arabidopsis thaliana (cs)
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| skos:notation
| - RIV/00216224:14310/04:00025491!RIV09-MSM-14310___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14310/04:00025491
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Arabidopsis; proteomics; cytokinin; 2-D electrophoresis (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - Chemica 43S Supplement (Collected Reports of the Natural Science Faculty Palacký University Olomouc Czech Republic)
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Konečná, Hana
- Zdráhal, Zbyněk
- Brzobohatý, Břetislav
- Lochmanová, Gabriela
- Váňová, Pavlína
- Chevalier, François
- Rossignol, Michel
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open.../riv/zahajeniAkce
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| http://linked.open...n/vavai/riv/zamer
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| number of pages
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| http://purl.org/ne...btex#hasPublisher
| - Univerzita Palackého v Olomouci
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| https://schema.org/isbn
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| http://localhost/t...ganizacniJednotka
| |
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