About: Occurrence of Borrelia afzelii and Borrelia garinii in Ixodes ricinus Ticks from Southern Moravia, Czech Republic     Goto   Sponge   NotDistinct   Permalink

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Description
  • During the years 1996 - 2000, a total of 2398 Ixodes ricinus ticks were collected in three areas of southern Moravia and eastern Bohemia, Czech Republic, and examined by dark-field microscopy for the presence of spirochetes. The prevalence of B. burgdorferi s. l. in Ixodes ricinus ticks varied and depended upon the year. In 1996 the prevalence 6.8 % was observed, during 1997 and 1998 it increased to 8.4 % and 12.3 %, respectively. The lowest prevalence was observed in 1999 (3.6 %) and in 2000 it increased to 4.0 %. The mean rate of infection was 6.5 % and the proportions of infected ticks were 12.2 % in 263 male ticks, 8.3 % in 289 female ticks, 6.0 % in 1621 nymphs and 1.3 % in 225 larvae. From the total of 156 highly infected ticks (>100 spirochetes per sample) transferred into BSK-H medium for isolation attempts, thirteen isolates were obtained. PCR-RFLP and electrophoresis (SDS-PAGE) were used for the identification and characterization of Borrelia strains. Ten tick isolates were identified as
  • During the years 1996 - 2000, a total of 2398 Ixodes ricinus ticks were collected in three areas of southern Moravia and eastern Bohemia, Czech Republic, and examined by dark-field microscopy for the presence of spirochetes. The prevalence of B. burgdorferi s. l. in Ixodes ricinus ticks varied and depended upon the year. In 1996 the prevalence 6.8 % was observed, during 1997 and 1998 it increased to 8.4 % and 12.3 %, respectively. The lowest prevalence was observed in 1999 (3.6 %) and in 2000 it increased to 4.0 %. The mean rate of infection was 6.5 % and the proportions of infected ticks were 12.2 % in 263 male ticks, 8.3 % in 289 female ticks, 6.0 % in 1621 nymphs and 1.3 % in 225 larvae. From the total of 156 highly infected ticks (>100 spirochetes per sample) transferred into BSK-H medium for isolation attempts, thirteen isolates were obtained. PCR-RFLP and electrophoresis (SDS-PAGE) were used for the identification and characterization of Borrelia strains. Ten tick isolates were identified as (en)
  • Přítomnost of Borrelia afzelii a Borrelia garinii v Ixodes ricinus na jižní Moravě, Czech Republic, PCR metoda (cs)
Title
  • Přítomnost of Borrelia afzelii a Borrelia garinii v Ixodes ricinus na jižní Moravě, Czech Republic (cs)
  • Occurrence of Borrelia afzelii and Borrelia garinii in Ixodes ricinus Ticks from Southern Moravia, Czech Republic
  • Occurrence of Borrelia afzelii and Borrelia garinii in Ixodes ricinus Ticks from Southern Moravia, Czech Republic (en)
skos:prefLabel
  • Přítomnost of Borrelia afzelii a Borrelia garinii v Ixodes ricinus na jižní Moravě, Czech Republic (cs)
  • Occurrence of Borrelia afzelii and Borrelia garinii in Ixodes ricinus Ticks from Southern Moravia, Czech Republic
  • Occurrence of Borrelia afzelii and Borrelia garinii in Ixodes ricinus Ticks from Southern Moravia, Czech Republic (en)
skos:notation
  • RIV/00216224:14310/04:00025485!RIV09-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z6093917), Z(MSM 143100008), Z(MSM 143100010)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 577359
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/04:00025485
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Ixodes ricinus Borrelia afzelii Borrelia garinii Prevalence Identification PCR-RFLP SDS-PAGE (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [861F91D22FC9]
http://linked.open...i/riv/nazevZdroje
  • Vector-Borne and Zoonotic Diseases
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 4
http://linked.open...iv/tvurceVysledku
  • Žákovská, Alena
  • Halouzka, Jiří
  • Dendis, Miloš
  • Janouškovcová, Eva
http://linked.open...n/vavai/riv/zamer
issn
  • 1530-3667
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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