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Description
  • Molekulárně-biologické metody (rodově a druhově specifická PCR a restrikční analýza amplifikované 16S rDNA s využitím 3 restrikčních enzymů) byly zvoleny pro ověření správnosti fenotypové identifikace izolátů bifidobakterií ze sbírky CCDM (Tábor, ČR). Molekulární metody byly zavedeny a optimalizovány s využitím typových a referenčních kultur bifidobakterií. Zvolené techniky umožnily identifikaci CCDM izolátů na úrověň druhů a poskytovaly shodné výsledky. (cs)
  • Two molecular methods were applied to verify the species identification of Bifidobacterium isolates from the Culture Collection of Dairy Microorganisms (CCDM, Tábor, ČR), previously identified by fenotypic techniques only. The methods used for identification in this study were the genus and species specific PCR and restriction analysis of amplified ribosomal DNA restriction analysis with three restriction enzymes. The methods were introduced and optimalised using type and reference strains of Bifidobacterium species. The molecular methods chosen enabled species identification of the analysed strains and provided congruent results.
  • Two molecular methods were applied to verify the species identification of Bifidobacterium isolates from the Culture Collection of Dairy Microorganisms (CCDM, Tábor, ČR), previously identified by fenotypic techniques only. The methods used for identification in this study were the genus and species specific PCR and restriction analysis of amplified ribosomal DNA restriction analysis with three restriction enzymes. The methods were introduced and optimalised using type and reference strains of Bifidobacterium species. The molecular methods chosen enabled species identification of the analysed strains and provided congruent results. (en)
Title
  • Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis
  • Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis (en)
  • Identifikace bifidobakterií s využitím metod PCR a ARDRA (cs)
skos:prefLabel
  • Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis
  • Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis (en)
  • Identifikace bifidobakterií s využitím metod PCR a ARDRA (cs)
skos:notation
  • RIV/00216224:14310/04:00019893!RIV08-GA0-14310___
http://linked.open.../vavai/riv/strany
  • 151
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA202/02/1361)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
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  • 567143
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  • RIV/00216224:14310/04:00019893
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Bifidobacterium spp.; identification; genus and species specific PCR; ARDRA (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [F5B31337B769]
http://linked.open...v/mistoKonaniAkce
  • Portorož, Slovenia
http://linked.open...i/riv/mistoVydani
  • Ljubljana, Slovenia
http://linked.open...i/riv/nazevZdroje
  • The 19th international ICFMH symposium FoodMicro 2004: New tools for improving microbial food safety and quality. Biotechnology and molecular biology approaches. 12-16th September 2004, Portorož, Slovenia
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Rittich, Bohuslav
  • Španová, Alena
  • Křížová, Jana
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
http://purl.org/ne...btex#hasPublisher
  • Slovenian Microbiology Society
https://schema.org/isbn
  • 961-90346-3-5
http://localhost/t...ganizacniJednotka
  • 14310
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