About: Analysis of phytochelatin (g-Glu-Cys)2-Gly by a multi-channel electrochemical detector in online connection with reversed-phase liquid chromatography     Goto   Sponge   NotDistinct   Permalink

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Description
  • Analýza fytochelatinu (g-Glu-Cys)2-Gly pomocí multikanálového elektrochemického detektoru v online napojení na HPLC s reverzní fází (cs)
  • Phytochelatins (PCs) are Cys-rich peptides produced by plant or some animal cells as protective molecules against heavy-metal ions in the cytoplasm. PCs have the basic formula (g-Glu-Cys)n-X (n: 2 to 11, X: terminal amino acid as Gly). PCs contain cysteine SH-groups and can form metal-PC complexes in cell environment. For the analysis of PCs different separation and detection methods were used, including electrochemical methods. In this work we present determination of PC2 using HPLC-ED. For this purpose hydrodynamic voltammetric measurements were used at a potential of 0.7 V giving the best current response. We tested the effect of temperature of the chromatographic column and the electrochemical detector on chromatographic separation. The best results were observed at 25 C. An HPLC-ED system consisted of two solvent delivery pumps and an eight-channel CoulArray electrochemical detector. This detector was located after the HPLC system with an XDB C18 reverse-phase column (mobile phase: trifluoroaceti
  • Phytochelatins (PCs) are Cys-rich peptides produced by plant or some animal cells as protective molecules against heavy-metal ions in the cytoplasm. PCs have the basic formula (g-Glu-Cys)n-X (n: 2 to 11, X: terminal amino acid as Gly). PCs contain cysteine SH-groups and can form metal-PC complexes in cell environment. For the analysis of PCs different separation and detection methods were used, including electrochemical methods. In this work we present determination of PC2 using HPLC-ED. For this purpose hydrodynamic voltammetric measurements were used at a potential of 0.7 V giving the best current response. We tested the effect of temperature of the chromatographic column and the electrochemical detector on chromatographic separation. The best results were observed at 25 C. An HPLC-ED system consisted of two solvent delivery pumps and an eight-channel CoulArray electrochemical detector. This detector was located after the HPLC system with an XDB C18 reverse-phase column (mobile phase: trifluoroaceti (en)
Title
  • Analysis of phytochelatin (g-Glu-Cys)2-Gly by a multi-channel electrochemical detector in online connection with reversed-phase liquid chromatography
  • Analysis of phytochelatin (g-Glu-Cys)2-Gly by a multi-channel electrochemical detector in online connection with reversed-phase liquid chromatography (en)
  • Analýza fytochelatinu (g-Glu-Cys)2-Gly pomocí multikanálového elektrochemického detektoru v online napojení na HPLC s reverzní fází (cs)
skos:prefLabel
  • Analysis of phytochelatin (g-Glu-Cys)2-Gly by a multi-channel electrochemical detector in online connection with reversed-phase liquid chromatography
  • Analysis of phytochelatin (g-Glu-Cys)2-Gly by a multi-channel electrochemical detector in online connection with reversed-phase liquid chromatography (en)
  • Analýza fytochelatinu (g-Glu-Cys)2-Gly pomocí multikanálového elektrochemického detektoru v online napojení na HPLC s reverzní fází (cs)
skos:notation
  • RIV/00216224:14310/04:00011237!RIV09-GA0-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA203/02/0422), P(IAA1163201)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 554248
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/04:00011237
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Reversed-phase liquid chromatography; electrochemical detector; phytochelatin; limit detection (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [120766AA58C4]
http://linked.open...v/mistoKonaniAkce
  • Thessaloniki. Greece
http://linked.open...i/riv/mistoVydani
  • Thessaloniki, Greece
http://linked.open...i/riv/nazevZdroje
  • Book of Abstracts I - 55th Annual Meeting of the ISE
http://linked.open...in/vavai/riv/obor
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http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
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http://linked.open...iv/tvurceVysledku
  • Adam, Vojtěch
  • Havel, Ladislav
  • Kizek, René
  • Trnková, Libuše
  • Zehnálek, Josef
  • Jelen, František
  • Klejdus, Bořivoj
  • Petrlová, Jitka
  • Potěšil, David
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
http://purl.org/ne...btex#hasPublisher
  • International Society of Electrochemistry
http://localhost/t...ganizacniJednotka
  • 14310
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