About: Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutathione in the maize (Zea mays L.) kernels exposed to cadmium.     Goto   Sponge   NotDistinct   Permalink

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  • Changes in fresh weight, overall protein amounts (Bradford method), cadmium concentration (DPASV) and glutathione content (HPLC/MS) were studied in maize kernels cultivated for five days at three different cadmium concentrations (0, 10 and 100 uM CdCl2). A highly sensitive HPLC/MS for determination of glutathione on a reversed-phase Atlantis dC18 chromatographic column (150 mm 2.1 mm, 3 um particle size) in an isocratic mode with acetonitrile - 0.01% TFA (5:95, flow rate 0.1 mL/min) was applied. Cadmium concentration was measured by differential pulse adsorptive stripping voltammetry (DPASV) after deposition on HMDE at potential --0.7 V (accumulation time 180 s, acetate buffer of pH 3.6) using an AUTOLAB with a VA-Stand 663 and a three-electrode system consisting of a hanging mercury drop electrode (HMDE) as a working electrode with area 0.4 mm2, an Ag/AgCl/3 M KCl as a reference electrode and a Pt-wire as an auxiliary electrode. Maize kernels exposed to the highest cadmium concentration (100 uM) germ
  • Changes in fresh weight, overall protein amounts (Bradford method), cadmium concentration (DPASV) and glutathione content (HPLC/MS) were studied in maize kernels cultivated for five days at three different cadmium concentrations (0, 10 and 100 uM CdCl2). A highly sensitive HPLC/MS for determination of glutathione on a reversed-phase Atlantis dC18 chromatographic column (150 mm 2.1 mm, 3 um particle size) in an isocratic mode with acetonitrile - 0.01% TFA (5:95, flow rate 0.1 mL/min) was applied. Cadmium concentration was measured by differential pulse adsorptive stripping voltammetry (DPASV) after deposition on HMDE at potential --0.7 V (accumulation time 180 s, acetate buffer of pH 3.6) using an AUTOLAB with a VA-Stand 663 and a three-electrode system consisting of a hanging mercury drop electrode (HMDE) as a working electrode with area 0.4 mm2, an Ag/AgCl/3 M KCl as a reference electrode and a Pt-wire as an auxiliary electrode. Maize kernels exposed to the highest cadmium concentration (100 uM) germ (en)
  • Changes in fresh weight, overall protein amounts (Bradford method), cadmium concentration (DPASV) and glutathione content (HPLC/MS) were studied in maize kernels cultivated for five days at three different cadmium concentrations (0, 10 and 100 uM CdCl2). A highly sensitive HPLC/MS for determination of glutathione on a reversed-phase Atlantis dC18 chromatographic column (150 mm 2.1 mm, 3 um particle size) in an isocratic mode with acetonitrile - 0.01% TFA (5:95, flow rate 0.1 mL/min) was applied. Cadmium concentration was measured by differential pulse adsorptive stripping voltammetry (DPASV) after deposition on HMDE at potential --0.7 V (accumulation time 180 s, acetate buffer of pH 3.6) using an AUTOLAB with a VA-Stand 663 and a three-electrode system consisting of a hanging mercury drop electrode (HMDE) as a working electrode with area 0.4 mm2, an Ag/AgCl/3 M KCl as a reference electrode and a Pt-wire as an auxiliary electrode. Maize kernels exposed to the highest cadmium concentration (100 uM) germ (cs)
Title
  • Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutathione in the maize (Zea mays L.) kernels exposed to cadmium.
  • Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutathione in the maize (Zea mays L.) kernels exposed to cadmium. (en)
  • HPLC/MS stanovení subpikomolárních koncentrací glutathionu v kukuřičných zrnech exponovaných kadmiu. (cs)
skos:prefLabel
  • Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutathione in the maize (Zea mays L.) kernels exposed to cadmium.
  • Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutathione in the maize (Zea mays L.) kernels exposed to cadmium. (en)
  • HPLC/MS stanovení subpikomolárních koncentrací glutathionu v kukuřičných zrnech exponovaných kadmiu. (cs)
skos:notation
  • RIV/00216224:14310/04:00011157!RIV08-MSM-14310___
http://linked.open.../vavai/riv/strany
  • 117-124
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(LN00A081), Z(MSM 432100001)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 588923
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/04:00011157
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • glutathione; HPLC/MS; voltammetry; Zea mays L. (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [3D700772A866]
http://linked.open...i/riv/nazevZdroje
  • Analytica Chimica Acta
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 520
http://linked.open...iv/tvurceVysledku
  • Adam, Vojtěch
  • Havel, Ladislav
  • Kizek, René
  • Kubáň, Vlastimil
  • Trnková, Libuše
  • Zehnálek, Josef
  • Vacek, Jan
  • Klejdus, Bořivoj
  • Petřek, Jiří
  • Rozik, Roland
http://linked.open...n/vavai/riv/zamer
issn
  • 0003-2670
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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