About: Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection.     Goto   Sponge   NotDistinct   Permalink

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Description
  • Water- and fat-soluble vitamins were separated on a MetaChem Polaris C18-A (150 mm x 4.6 mm, 3 mum particle size) in a single run using combined isocratic and linear gradient elution with a mobile phase consisting of 0.010% trifluoroacetic acid of pH 3.9 (solvent A) and methanol (solvent B) at the flow rate 0.7 ml min(-1). A linear gradient profile (A:B) started at 95:5 and was constant in the first 4 min, then linearly decreased up to 2:98 during the next 6 min, then it was constant in the next 20 min and finally linearly increased up to 95:5 ratio of water phase in the last 5 min of the separation. The most suitable detection wavelength for simultaneous vitamin determination was 280 nm. The method was applied for the solid sample of pharmaceutical preparation (B-Komplex), fortified powdered drinks (multi-vitamin) and food samples. The results were in good agreement with the declared values.
  • Water- and fat-soluble vitamins were separated on a MetaChem Polaris C18-A (150 mm x 4.6 mm, 3 mum particle size) in a single run using combined isocratic and linear gradient elution with a mobile phase consisting of 0.010% trifluoroacetic acid of pH 3.9 (solvent A) and methanol (solvent B) at the flow rate 0.7 ml min(-1). A linear gradient profile (A:B) started at 95:5 and was constant in the first 4 min, then linearly decreased up to 2:98 during the next 6 min, then it was constant in the next 20 min and finally linearly increased up to 95:5 ratio of water phase in the last 5 min of the separation. The most suitable detection wavelength for simultaneous vitamin determination was 280 nm. The method was applied for the solid sample of pharmaceutical preparation (B-Komplex), fortified powdered drinks (multi-vitamin) and food samples. The results were in good agreement with the declared values. (en)
  • Water- and fat-soluble vitamins were separated on a MetaChem Polaris C18-A (150 mm x 4.6 mm, 3 mum particle size) in a single run using combined isocratic and linear gradient elution with a mobile phase consisting of 0.010% trifluoroacetic acid of pH 3.9 (solvent A) and methanol (solvent B) at the flow rate 0.7 ml min(-1). A linear gradient profile (A:B) started at 95:5 and was constant in the first 4 min, then linearly decreased up to 2:98 during the next 6 min, then it was constant in the next 20 min and finally linearly increased up to 95:5 ratio of water phase in the last 5 min of the separation. The most suitable detection wavelength for simultaneous vitamin determination was 280 nm. The method was applied for the solid sample of pharmaceutical preparation (B-Komplex), fortified powdered drinks (multi-vitamin) and food samples. The results were in good agreement with the declared values. (cs)
Title
  • Současné stanovení vitamínů rozpustných ve vodě a v tucích metodou HPLC (cs)
  • Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection.
  • Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection. (en)
skos:prefLabel
  • Současné stanovení vitamínů rozpustných ve vodě a v tucích metodou HPLC (cs)
  • Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection.
  • Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection. (en)
skos:notation
  • RIV/00216224:14310/04:00011152!RIV09-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM 432100001)
http://linked.open...iv/cisloPeriodika
  • 1-2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 586354
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/04:00011152
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • diode array detection; drugs; fat-soluble vitamins; high-performance liquid chromatography; mass spectrometry; pharmaceutical preparations; simultaneous determination; vitamins; water-soluble vitamins (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [C65980599F60]
http://linked.open...i/riv/nazevZdroje
  • Analytica Chimica Acta
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 520
http://linked.open...iv/tvurceVysledku
  • Adam, Vojtěch
  • Kizek, René
  • Kubáň, Vlastimil
  • Vacek, Jan
  • Klejdus, Bořivoj
  • Petrlová, Jitka
  • Mikelová, Radka
  • Potěšil, David
http://linked.open...n/vavai/riv/zamer
issn
  • 0003-2670
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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