About: Esterases as a marker for growth of BY-2 tobacco cells and early somatic embryos of the Norway spruce.     Goto   Sponge   NotDistinct   Permalink

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Description
  • Growth is one of the basic properties of biological systems. The methods which are commonly used for the determination of growth are usually difficult and not very accurate. In the present work we decided to use esterase activity as a growth marker in tobacco suspension culture (BY-2 line) and in early somatic embryos of Norway spruce (clone 2/32) grown on a semi-solid medium. Esterase activity correlates well with the classical growth characteristics of BY-2 and spruce early somatic embryos. Determination of esterase activity is based on spectrophotometric and spectrofluorimetric detection of reaction products, which arise from the enzymatic hydrolysis of two substrates (p-nitrophenyl acetate and fluorescein diacetate) by esterase. The spectrophotometric method enabled us to detect approximately 10(4) BY-2 cells and 25 spruce embryos whereas the more sensitive spectrofluorimetric method allowed us to detect approximately 800 BY-2 cells and 5 early somatic embryos of Norway spruce.
  • Growth is one of the basic properties of biological systems. The methods which are commonly used for the determination of growth are usually difficult and not very accurate. In the present work we decided to use esterase activity as a growth marker in tobacco suspension culture (BY-2 line) and in early somatic embryos of Norway spruce (clone 2/32) grown on a semi-solid medium. Esterase activity correlates well with the classical growth characteristics of BY-2 and spruce early somatic embryos. Determination of esterase activity is based on spectrophotometric and spectrofluorimetric detection of reaction products, which arise from the enzymatic hydrolysis of two substrates (p-nitrophenyl acetate and fluorescein diacetate) by esterase. The spectrophotometric method enabled us to detect approximately 10(4) BY-2 cells and 25 spruce embryos whereas the more sensitive spectrofluorimetric method allowed us to detect approximately 800 BY-2 cells and 5 early somatic embryos of Norway spruce. (en)
  • Growth is one of the basic properties of biological systems. The methods which are commonly used for the determination of growth are usually difficult and not very accurate. In the present work we decided to use esterase activity as a growth marker in tobacco suspension culture (BY-2 line) and in early somatic embryos of Norway spruce (clone 2/32) grown on a semi-solid medium. Esterase activity correlates well with the classical growth characteristics of BY-2 and spruce early somatic embryos. Determination of esterase activity is based on spectrophotometric and spectrofluorimetric detection of reaction products, which arise from the enzymatic hydrolysis of two substrates (p-nitrophenyl acetate and fluorescein diacetate) by esterase. The spectrophotometric method enabled us to detect approximately 10(4) BY-2 cells and 25 spruce embryos whereas the more sensitive spectrofluorimetric method allowed us to detect approximately 800 BY-2 cells and 5 early somatic embryos of Norway spruce. (cs)
Title
  • Esterases as a marker for growth of BY-2 tobacco cells and early somatic embryos of the Norway spruce. (en)
  • Esterasy jako marker růstu u BY-2 buněk tabáku a raných somatických embryí smrku ztepilého
  • Esterasy jako marker růstu u BY-2 buněk tabáku a raných somatických embryí smrku ztepilého (cs)
skos:prefLabel
  • Esterases as a marker for growth of BY-2 tobacco cells and early somatic embryos of the Norway spruce. (en)
  • Esterasy jako marker růstu u BY-2 buněk tabáku a raných somatických embryí smrku ztepilého
  • Esterasy jako marker růstu u BY-2 buněk tabáku a raných somatických embryí smrku ztepilého (cs)
skos:notation
  • RIV/00216224:14310/04:00011141!RIV08-MSM-14310___
http://linked.open.../vavai/riv/strany
  • 195-201
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(LN00A081)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 563148
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/04:00011141
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • BY-2; cell viability; early somatic embryo; esterase; fluorescein diacetate; Nicotiana tabacum; Picea abies (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [1F543F2A94C8]
http://linked.open...i/riv/nazevZdroje
  • Plant cell tissue and organ culture
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 97
http://linked.open...iv/tvurceVysledku
  • Adam, Vojtěch
  • Havel, Ladislav
  • Kizek, René
  • Vacek, Jan
  • Víteček, Jan
  • Petřek, Jiří
issn
  • 0167-6857
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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