About: Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells     Goto   Sponge   NotDistinct   Permalink

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  • Nitric oxide is a denitrification intermediate which is produced from nitrite and then further converted via nitrous oxide to nitrogen. Here, the effect of low concentrations of the protonophore carbonylcyanide m-chlorophenylhydrazone on the time courses for dissolved gases was examined. While NO was found to oscillate, N(2)O only increased gradually as the reduction of nitrite progressed. The frequency and shape of protonophore-induced NO oscillations were influenced by temperature and the concentration of electron donor N,N,N',N'-tetramethyl-p-phenylene diamine (TMPD) in a manner compatible with the observed differential effects on the two involved enzyme activities. We demonstrated the existence of a pH interval, where [NO] oscillates even without uncoupler addition. Occurrence of nitric oxide oscillations in mixtures of a nitrite reductase mutant with a nitric oxide reductase mutant suggests that they cannot be due to a competition of the enzymes for redox equivalents from one common respiratory c
  • Nitric oxide is a denitrification intermediate which is produced from nitrite and then further converted via nitrous oxide to nitrogen. Here, the effect of low concentrations of the protonophore carbonylcyanide m-chlorophenylhydrazone on the time courses for dissolved gases was examined. While NO was found to oscillate, N(2)O only increased gradually as the reduction of nitrite progressed. The frequency and shape of protonophore-induced NO oscillations were influenced by temperature and the concentration of electron donor N,N,N',N'-tetramethyl-p-phenylene diamine (TMPD) in a manner compatible with the observed differential effects on the two involved enzyme activities. We demonstrated the existence of a pH interval, where [NO] oscillates even without uncoupler addition. Occurrence of nitric oxide oscillations in mixtures of a nitrite reductase mutant with a nitric oxide reductase mutant suggests that they cannot be due to a competition of the enzymes for redox equivalents from one common respiratory c (en)
  • Oxid dusnatý je meziprodukt denitrifikace, který vzniká z dusitanu a je dále přeměňován přes oxid dusný na dusík. Byl sledován vliv nízkých koncentrací protonoforu karbonylkyanid-m-chlorfenylhydrazonu na časový průběh koncentrací rozpuštěných plynů. Zatímco u NO byly zjištěny oscilace, N2O se postupně hromadil. Frekvence a tvar oscilací NO vyvolaných protonoforem byly ovlivňovány teplotou a koncentrací elektronového donoru TMPD v souladu s pozorovaným působením na aktivity jednotlivých enzymů. Ukázali jsme existenci intervalu pH ve kterém NO osciluje dokonce bez přidání rozpojovače. Výskyt oscilací NO ve směsi mutant ukazuje, že nemohou být způsobeny kompeticí obou enzymů o redukční ekvivalenty z jednoho společného respiračního řetězce. (cs)
Title
  • Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells
  • Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells (en)
  • Oscilace oxidu dusnatého u Paracoccus denitrificans: vliv faktorů prostředí o oddělení nitritreduktasy a reduktasy oxidu dusnatého do různých buněk (cs)
skos:prefLabel
  • Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells
  • Nitric oxide oscillations in Paracoccus denitrificans: the effects of environmental factors and of segregating nitrite reductase and nitric oxide reductase into separate cells (en)
  • Oscilace oxidu dusnatého u Paracoccus denitrificans: vliv faktorů prostředí o oddělení nitritreduktasy a reduktasy oxidu dusnatého do různých buněk (cs)
skos:notation
  • RIV/00216224:14310/04:00010556!RIV08-MSM-14310___
http://linked.open.../vavai/riv/strany
  • 237-243
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM 143100005)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 576279
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/04:00010556
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • denitrification; nitrite reductase; nitric oxide reductase; substrate inhibition; concentration oscillations (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [814C61BCE0CC]
http://linked.open...i/riv/nazevZdroje
  • Archives of biochemistry and biophysics
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 429
http://linked.open...iv/tvurceVysledku
  • Kučera, Igor
  • Van Spanning, Rob J. M.
  • Kuňák, Michal
http://linked.open...n/vavai/riv/zamer
issn
  • 0003-9861
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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