About: Study of enzyme kinetics of phenol sulfotransferase by electrophoretically mediated microanalysis

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About: Study of enzyme kinetics of phenol sulfotransferase by electrophoretically mediated microanalysis Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Metoda EMMA byla využita pro studium enzymove kinetiky enzymu fenolsulfotranferasy. (cs) Electrophoretically mediated microanalysis (EMMA) was applied for the study of the kinetic parameters of the enzymatic reaction of phenol sulfotransferase SULT1A1 isoenzyme with 4-nitrophenol as a substrate. The SULT1A1 activity was determined by the quantitation of the product, 4-nitrophenyl sulfate, at 274 nm by using different injection and separation steps. This new approach solved the problem of the presence of the very strong inhibitor, adenosine 3',5'-bisphosphate (PAP), in the co-substrate solution (adenosine 3'-phosphate 5'-phosphosulfate, PAPS) which is unstable at room temperature. The inhibitor PAP was electrophoretically separated from the co-substrate PAPS before the injection of enzyme and substrate inside the capillary (and thus before their in-capillary encountering). With the developed in-capillary SULT1A1 activity assay an average Michaelis constant (Km) for 4-nitrophenol was calculated to be 0.84 M, a value which is consistent with a previously reported value. Strong substrate inhi Electrophoretically mediated microanalysis (EMMA) was applied for the study of the kinetic parameters of the enzymatic reaction of phenol sulfotransferase SULT1A1 isoenzyme with 4-nitrophenol as a substrate. The SULT1A1 activity was determined by the quantitation of the product, 4-nitrophenyl sulfate, at 274 nm by using different injection and separation steps. This new approach solved the problem of the presence of the very strong inhibitor, adenosine 3',5'-bisphosphate (PAP), in the co-substrate solution (adenosine 3'-phosphate 5'-phosphosulfate, PAPS) which is unstable at room temperature. The inhibitor PAP was electrophoretically separated from the co-substrate PAPS before the injection of enzyme and substrate inside the capillary (and thus before their in-capillary encountering). With the developed in-capillary SULT1A1 activity assay an average Michaelis constant (Km) for 4-nitrophenol was calculated to be 0.84 M, a value which is consistent with a previously reported value. Strong substrate inhi (en)
Title	Studium enzymove kinetiky enzymu fenolsulfotranferasy metodou EMMA (cs) Study of enzyme kinetics of phenol sulfotransferase by electrophoretically mediated microanalysis Study of enzyme kinetics of phenol sulfotransferase by electrophoretically mediated microanalysis (en)
skos:prefLabel	Studium enzymove kinetiky enzymu fenolsulfotranferasy metodou EMMA (cs) Study of enzyme kinetics of phenol sulfotransferase by electrophoretically mediated microanalysis Study of enzyme kinetics of phenol sulfotransferase by electrophoretically mediated microanalysis (en)
skos:notation	RIV/00216224:14310/04:00009928!RIV09-GA0-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/03/1025), P(GA203/03/1125), Z(MSM 143100005)
http://linked.open...iv/cisloPeriodika	(1+2)
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Vytisková, Soňa Glatz, Zdeněk
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	588780
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/04:00009928
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Capillary electrophoresis; enzymes; EMMA; sulfotranferase (en)
http://linked.open.../riv/klicoveSlovo	Capillary electrophoresis enzymes sulfotranferase EMMA
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[30E5698F3DF3]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Use of the real-time PCR for fetal DNA analysis in maternal plasma Application of capillary zone electrophoresis for the study of enzymes
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	1032
http://linked.open...iv/tvurceVysledku	Glatz, Zdeněk Vytisková, Soňa Hoogmartens, Jos Van Schepdael, Ann Van Dyck, Sigrid
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20143100005
issn	0021-9606
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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