About: Re-construction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinB     Goto   Sponge   NotDistinct   Permalink

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Description
  • Model proteinu Rv2579 z bakterie Mycobacretium tuberculosis byl srovnán s krystalovou strukturou haloalkán dehalogenázy LinB z bakterie Sphingomonas paucimobilis. (cs)
  • The homology model of protein Rv2579 from Mycobacterium tuberculosis H37Rv was compared with the crystal structure of haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26 and uncovered that six out of nineteen amino acid residues which form an active site and entrance tunnel are different between LinB and Rv2579. To characterize an effect of replacements of these six amino acid residues, mutations were introduced cumulatively into the six amino acid residues of LinB. The six-fold mutant which is supposed to have an active site of Rv2579 showed haloalkane dehalogenase activity with tested haloalkanes, confirming that Rv2579 is a member of haloalkane dehalogenase protein family.
  • The homology model of protein Rv2579 from Mycobacterium tuberculosis H37Rv was compared with the crystal structure of haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26 and uncovered that six out of nineteen amino acid residues which form an active site and entrance tunnel are different between LinB and Rv2579. To characterize an effect of replacements of these six amino acid residues, mutations were introduced cumulatively into the six amino acid residues of LinB. The six-fold mutant which is supposed to have an active site of Rv2579 showed haloalkane dehalogenase activity with tested haloalkanes, confirming that Rv2579 is a member of haloalkane dehalogenase protein family. (en)
Title
  • Re-construction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinB
  • Re-construction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinB (en)
  • Re-konstrukce mycobakteriální dehalogenázy Rv2579 kumulativní mutagenezí haloalkán dehalogenázy LinB (cs)
skos:prefLabel
  • Re-construction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinB
  • Re-construction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinB (en)
  • Re-konstrukce mycobakteriální dehalogenázy Rv2579 kumulativní mutagenezí haloalkán dehalogenázy LinB (cs)
skos:notation
  • RIV/00216224:14310/03:00009851!RIV08-MSM-14310___
http://linked.open.../vavai/riv/strany
  • 2349-2355
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(LN00A016)
http://linked.open...iv/cisloPeriodika
  • 4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 624839
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/03:00009851
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • MUTANT; EVOLUTION; MYCOBACTERIUM; DEHALOGENATION; BIODEGRADATION (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [2A2A7270D7A5]
http://linked.open...i/riv/nazevZdroje
  • Applied and Environmental Microbiology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 69
http://linked.open...iv/tvurceVysledku
  • Damborský, Jiří
  • Nagata, Yuji
  • Prokop, Zbyněk
  • Sýkorová, Jana
  • Monincová, Marta
  • Marvanová, Soňa
  • Tsuda, Masataka
issn
  • 1098-5336
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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