About: Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2)

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About: Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2) Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The second internal transcribed spacer (ITS2) of ribosomal DNA of diplozoid parasites was amplified, sequenced and restriction enzyme digestion was performed to reveal restriction fragment length polymorphism (RFLP). Genetic markers for 8 species of European diplozoids were developed. As those diplozoids are very closely related, it was very difficult to find suitable enzymes to reveal different patterns for each species. Only a combination of digestion with AluI, HaeIII, HinfI, RsaI and SphI proved convenient to discriminate all species studied. Morphological analysis and measurements of the attachment apparatus of the studied species are also presented, together with comparison of the shape of trapeze spur and the anterior joining sclerites. The second internal transcribed spacer (ITS2) of ribosomal DNA of diplozoid parasites was amplified, sequenced and restriction enzyme digestion was performed to reveal restriction fragment length polymorphism (RFLP). Genetic markers for 8 species of European diplozoids were developed. As those diplozoids are very closely related, it was very difficult to find suitable enzymes to reveal different patterns for each species. Only a combination of digestion with AluI, HaeIII, HinfI, RsaI and SphI proved convenient to discriminate all species studied. Morphological analysis and measurements of the attachment apparatus of the studied species are also presented, together with comparison of the shape of trapeze spur and the anterior joining sclerites. (en)
Title	Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2) Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2) (en)
skos:prefLabel	Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2) Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2) (en)
skos:notation	RIV/00216224:14310/03:00008781!RIV/2004/MSM/143104/N
http://linked.open.../vavai/riv/strany	44-45
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 143100010)
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Matějusová, Iveta Koubková, Božena
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	609664
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/03:00008781
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	monogenea, diplozoids, PCR, RFLP, dignostic (en)
http://linked.open.../riv/klicoveSlovo	monogenea PCR dignostic RFLP diplozoids
http://linked.open...ontrolniKodProRIV	[1AE804AA7A53]
http://linked.open...v/mistoKonaniAkce	Bergen, Norway
http://linked.open...i/riv/mistoVydani	Bergen, Norway
http://linked.open...i/riv/nazevZdroje	Bulletin of the Scandinavian Society for Parasitology, Proceedings of the XXI. Symposium of the Scandinavian Socity for Parasitology, Bergen, Norway 12-15 June, 2003
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2003
http://linked.open...iv/tvurceVysledku	Koubková, Božena Matějusová, Iveta Cunningham, Carey O.
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2003-01-01 (xsd:date)
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20143100010
issn	0803-4907
number of pages	2 (xsd:int)
http://purl.org/ne...btex#hasPublisher	HS-Trykk A/S, Norway
http://localhost/t...ganizacniJednotka	14310

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