About: Capillary Array Electrophoresis-MALDI Mass Spectrometry Using a Vacuum Deposition Interface     Goto   Sponge   NotDistinct   Permalink

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  • The approach of vacuum deposition has been extended to demonstrate parallel analysis for multiple on-line infusion MALDI TOF MS and capillary array electrophoresis (CAE)-MALDI MS. In the infusion mode, individual peptide samples were simultaneously deposited on a Mylar tape cartridge using an array of eight capillaries, yielding eight parallel traces. For CAE-MALDI/TOF MS, the same number of separation capillaries were coupled with an array of eight infusion capillaries using a common liquid junction, containing matrix solution. A fast-scanning mirror was employed to traverse the beam of the desorption laser across the Mylar tape to probe one trace at a time. The positions of the eight sample traces formed on the tape were automatically determined, and all samples were analyzed in rapid sequence using a kilohertz repetition rate laser and a high-throughput data acquisition system. The instrumentation was operated with CAE MS for high-throughput analysis without compromising data quality applicable to
  • The approach of vacuum deposition has been extended to demonstrate parallel analysis for multiple on-line infusion MALDI TOF MS and capillary array electrophoresis (CAE)-MALDI MS. In the infusion mode, individual peptide samples were simultaneously deposited on a Mylar tape cartridge using an array of eight capillaries, yielding eight parallel traces. For CAE-MALDI/TOF MS, the same number of separation capillaries were coupled with an array of eight infusion capillaries using a common liquid junction, containing matrix solution. A fast-scanning mirror was employed to traverse the beam of the desorption laser across the Mylar tape to probe one trace at a time. The positions of the eight sample traces formed on the tape were automatically determined, and all samples were analyzed in rapid sequence using a kilohertz repetition rate laser and a high-throughput data acquisition system. The instrumentation was operated with CAE MS for high-throughput analysis without compromising data quality applicable to (en)
Title
  • Capillary Array Electrophoresis-MALDI Mass Spectrometry Using a Vacuum Deposition Interface
  • Capillary Array Electrophoresis-MALDI Mass Spectrometry Using a Vacuum Deposition Interface (en)
skos:prefLabel
  • Capillary Array Electrophoresis-MALDI Mass Spectrometry Using a Vacuum Deposition Interface
  • Capillary Array Electrophoresis-MALDI Mass Spectrometry Using a Vacuum Deposition Interface (en)
skos:notation
  • RIV/00216224:14310/02:00008542!RIV/2004/GA0/143104/N
http://linked.open.../vavai/riv/strany
  • 17
http://linked.open...avai/riv/aktivita
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  • P(GA203/03/0515)
http://linked.open...iv/cisloPeriodika
  • 1
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  • 640085
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  • RIV/00216224:14310/02:00008542
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  • capillary array electrophoresis, MALDI, mass spectrometry, proteomics, peptide (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [2CEE08B88584]
http://linked.open...i/riv/nazevZdroje
  • Analytical Chemistry
http://linked.open...in/vavai/riv/obor
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 74
http://linked.open...iv/tvurceVysledku
  • Preisler, Jan
  • Hu, Ping
  • Rejtar, Tomáš
  • Karger, Barry L.
  • Moskoveets, Eugene
issn
  • 0003-2700
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http://localhost/t...ganizacniJednotka
  • 14310
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