About: Comparison of MRSA-screen latex agglutination, conventional phenotypic methods and <I>mecA</I> gene detection for identification of oxacilin resistance in staphylococci     Goto   Sponge   NotDistinct   Permalink

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  • On the whole, 128 staphylococcal isolates (Staphylococcus aureus 65, Staphylococcus epidermidis 42, and Staphylococcus haemolyticus 21 strains) from patients of ten Czech hospitals were classified as susceptible or resistant to oxacillin by the standard broth dilution micro-method (using the MIC break-point of 4.0 mg/L for resistance). The MRSA-Screen test (Denka Seiken Co., Japan), a latex agglutination for the detection of penicillin binding protein PBP2a in oxacillin/methicillin-resistant staphylococci, was compared with other commonly used phenotypic susceptibility tests. The PCR detection of the mecA gene, the presence of which is equaled to oxacillin resistance, was used as a reference genotypic method. In addition, the determination of the femA gene was performed also by this procedure. All 68 isolates (S. aureus 26, S. epidermidis 21, and S. haemolyticus 21 strains), determined as oxacillin-resistant, contained the mecA gene. The femA gene was present in all these S. aureus strains. The other
  • On the whole, 128 staphylococcal isolates (Staphylococcus aureus 65, Staphylococcus epidermidis 42, and Staphylococcus haemolyticus 21 strains) from patients of ten Czech hospitals were classified as susceptible or resistant to oxacillin by the standard broth dilution micro-method (using the MIC break-point of 4.0 mg/L for resistance). The MRSA-Screen test (Denka Seiken Co., Japan), a latex agglutination for the detection of penicillin binding protein PBP2a in oxacillin/methicillin-resistant staphylococci, was compared with other commonly used phenotypic susceptibility tests. The PCR detection of the mecA gene, the presence of which is equaled to oxacillin resistance, was used as a reference genotypic method. In addition, the determination of the femA gene was performed also by this procedure. All 68 isolates (S. aureus 26, S. epidermidis 21, and S. haemolyticus 21 strains), determined as oxacillin-resistant, contained the mecA gene. The femA gene was present in all these S. aureus strains. The other (en)
  • On the whole, 128 staphylococcal isolates (Staphylococcus aureus 65, Staphylococcus epidermidis 42, and Staphylococcus haemolyticus 21 strains) from patients of ten Czech hospitals were classified as susceptible or resistant to oxacillin by the standard broth dilution micro-method (using the MIC break-point of 4.0 mg/L for resistance). The MRSA-Screen test (Denka Seiken Co., Japan), a latex agglutination for the detection of penicillin binding protein PBP2a in oxacillin/methicillin-resistant staphylococci, was compared with other commonly used phenotypic susceptibility tests. The PCR detection of the mecA gene, the presence of which is equaled to oxacillin resistance, was used as a reference genotypic method. In addition, the determination of the femA gene was performed also by this procedure. All 68 isolates (S. aureus 26, S. epidermidis 21, and S. haemolyticus 21 strains), determined as oxacillin-resistant, contained the mecA gene. The femA gene was present in all these S. aureus strains. The other (cs)
Title
  • Comparison of MRSA-screen latex agglutination, conventional phenotypic methods and <I>mecA</I> gene detection for identification of oxacilin resistance in staphylococci
  • Comparison of MRSA-screen latex agglutination, conventional phenotypic methods and <I>mecA</I> gene detection for identification of oxacilin resistance in staphylococci (en)
  • Comparison of MRSA-screen latex agglutination, conventional phenotypic methods and <I>mecA</I> gene detection for identification of oxacilin resistance in staphylococci (cs)
skos:prefLabel
  • Comparison of MRSA-screen latex agglutination, conventional phenotypic methods and <I>mecA</I> gene detection for identification of oxacilin resistance in staphylococci
  • Comparison of MRSA-screen latex agglutination, conventional phenotypic methods and <I>mecA</I> gene detection for identification of oxacilin resistance in staphylococci (en)
  • Comparison of MRSA-screen latex agglutination, conventional phenotypic methods and <I>mecA</I> gene detection for identification of oxacilin resistance in staphylococci (cs)
skos:notation
  • RIV/00216224:14310/02:00007137!RIV09-GA0-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA301/02/1505), P(GA301/99/D075), Z(MSM 143100008)
http://linked.open...iv/cisloPeriodika
  • 6
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
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http://linked.open...dnocenehoVysledku
  • 641314
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/02:00007137
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Bacterial Typing Techniques; DNA; Staphylococcus aureus; Coagulase-Negative Staphylococci; Methicillin/Oxacillin Resistance; Screen Latex Agglutination (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • SK - Slovenská republika
http://linked.open...ontrolniKodProRIV
  • [7327DFA8900F]
http://linked.open...i/riv/nazevZdroje
  • Biologia, Section: Cellular and Molecular Biology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 57
http://linked.open...iv/tvurceVysledku
  • Doškař, Jiří
  • Kolář, Milan
  • Pantůček, Roman
  • Hájek, Václav
  • Rosypal, Stanislav
http://linked.open...ain/vavai/riv/wos
  • 000180726700011
http://linked.open...n/vavai/riv/zamer
issn
  • 1335-6399
number of pages
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  • 14310
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