About: Ergosterol induces mobilization of internal calcium in tobacco cells

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Description	Ergosterol interacts with tobacco suspension cells and triggers pH changes of extracellular medium, oxidative burst and synthesis of phytoalexins. Cryptogein stimulated an apparent continuous uptake of external calcium within 40 min, whereas no net uptake of external calcium occurred upon the addition of ergosterol. However, the elicitation with both cryptogein and ergosterol resulted in an increase of the fluorescence of calcium green 1 in cytosol. The use of several inhibitors of calcium channels (La3+, TMB-8, verapamil, ruthenium red, nifedipine) and a protein-kinase inhibitor (staurosporin) suggests that the elicitation with ergosterol includes the mobilization of internal calcium stores in vacuoles mediated by IP3 and serine/threonine protein kinases. Ergosterol interacts with tobacco suspension cells and triggers pH changes of extracellular medium, oxidative burst and synthesis of phytoalexins. Cryptogein stimulated an apparent continuous uptake of external calcium within 40 min, whereas no net uptake of external calcium occurred upon the addition of ergosterol. However, the elicitation with both cryptogein and ergosterol resulted in an increase of the fluorescence of calcium green 1 in cytosol. The use of several inhibitors of calcium channels (La3+, TMB-8, verapamil, ruthenium red, nifedipine) and a protein-kinase inhibitor (staurosporin) suggests that the elicitation with ergosterol includes the mobilization of internal calcium stores in vacuoles mediated by IP3 and serine/threonine protein kinases. (en) Ergosterol interacts with tobacco suspension cells and triggers pH changes of extracellular medium, oxidative burst and synthesis of phytoalexins. Cryptogein stimulated an apparent continuous uptake of external calcium within 40 min, whereas no net uptake of external calcium occurred upon the addition of ergosterol. However, the elicitation with both cryptogein and ergosterol resulted in an increase of the fluorescence of calcium green 1 in cytosol. The use of several inhibitors of calcium channels (La3+, TMB-8, verapamil, ruthenium red, nifedipine) and a protein-kinase inhibitor (staurosporin) suggests that the elicitation with ergosterol includes the mobilization of internal calcium stores in vacuoles mediated by IP3 and serine/threonine protein kinases. (cs)
Title	Ergosterol induces mobilization of internal calcium in tobacco cells Ergosterol induces mobilization of internal calcium in tobacco cells (en) Ergosterol induces mobilization of internal calcium in tobacco cells (cs)
skos:prefLabel	Ergosterol induces mobilization of internal calcium in tobacco cells Ergosterol induces mobilization of internal calcium in tobacco cells (en) Ergosterol induces mobilization of internal calcium in tobacco cells (cs)
skos:notation	RIV/00216224:14310/02:00006495!RIV08-MSM-14310___
http://linked.open.../vavai/riv/strany	91
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA522/02/0925), P(ME 321), P(NF5991), Z(MSM 143100005)
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Kašparovský, Tomáš Mikeš, Vladimír
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	645229
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/02:00006495
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ergposterol; plant protection; tobacco; elicitation (en)
http://linked.open.../riv/klicoveSlovo	elicitation ergposterol plant protection tobacco
http://linked.open...ontrolniKodProRIV	[70293B3440F3]
http://linked.open...v/mistoKonaniAkce	8 - 14 September 2002, Prague
http://linked.open...i/riv/mistoVydani	Prague
http://linked.open...i/riv/nazevZdroje	Book of Abstracts, EFPP Congress, Prague
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Genetic study of alcohol dependence : detection of the association between candidate gene polymorphisms and alcoholism. http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20321 Modeling of the structure and interaction of elicitors of the fungus Phytophthora
http://linked.open...UplatneniVysledku	2002
http://linked.open...iv/tvurceVysledku	Kašparovský, Tomáš Mikeš, Vladimír Milat, M. L. Blein, J. P.
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2002-01-01 (xsd:date)
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20143100005
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	European Foundation for Plant Pathology
http://localhost/t...ganizacniJednotka	14310

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