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| rdf:type
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| Description
| - In facultative anaerobic bacterium P. denitrificans, a broad range of substrates can serve as carbon sources and terminal electrone acceptors. Successfull adaptation of this bacterium to altered conditions depends on expression of metabolically active proteins. For example, nitrate induces expression of some denitrification enzymes. Many important redox proteins are membrane-associated. For comprehensive study on changes of proteins level in this bacterium, we have optimized a method using two-dimensional electrophoresis (2-DE). Our work represents the first analysis of P. denitrificans proteome using 2-DE. Membrane suspension of anaerobically grown cells of Paracoccus denitrificans was prepared by combination of enzymatic and osmotic lysis, centrifugation and ulgtracentrifugation. We have tested various detergents and procedures for membrane proteins presolubilization (e.g., SDS, CHAPS, dodecyl-â-D-maltoside with 6-aminocaproic acid). Sample buffer and IEF gel composition effects on 2-D gel patterns
- In facultative anaerobic bacterium P. denitrificans, a broad range of substrates can serve as carbon sources and terminal electrone acceptors. Successfull adaptation of this bacterium to altered conditions depends on expression of metabolically active proteins. For example, nitrate induces expression of some denitrification enzymes. Many important redox proteins are membrane-associated. For comprehensive study on changes of proteins level in this bacterium, we have optimized a method using two-dimensional electrophoresis (2-DE). Our work represents the first analysis of P. denitrificans proteome using 2-DE. Membrane suspension of anaerobically grown cells of Paracoccus denitrificans was prepared by combination of enzymatic and osmotic lysis, centrifugation and ulgtracentrifugation. We have tested various detergents and procedures for membrane proteins presolubilization (e.g., SDS, CHAPS, dodecyl-â-D-maltoside with 6-aminocaproic acid). Sample buffer and IEF gel composition effects on 2-D gel patterns (en)
- In facultative anaerobic bacterium P. denitrificans, a broad range of substrates can serve as carbon sources and terminal electrone acceptors. Successfull adaptation of this bacterium to altered conditions depends on expression of metabolically active proteins. For example, nitrate induces expression of some denitrification enzymes. Many important redox proteins are membrane-associated. For comprehensive study on changes of proteins level in this bacterium, we have optimized a method using two-dimensional electrophoresis (2-DE). Our work represents the first analysis of P. denitrificans proteome using 2-DE. Membrane suspension of anaerobically grown cells of Paracoccus denitrificans was prepared by combination of enzymatic and osmotic lysis, centrifugation and ulgtracentrifugation. We have tested various detergents and procedures for membrane proteins presolubilization (e.g., SDS, CHAPS, dodecyl-â-D-maltoside with 6-aminocaproic acid). Sample buffer and IEF gel composition effects on 2-D gel patterns (cs)
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| Title
| - Analysis of membrane proteome of Paracoccus denitrificans by two-dimensional electrophoresis
- Analysis of membrane proteome of Paracoccus denitrificans by two-dimensional electrophoresis (en)
- Analysis of membrane proteome of Paracoccus denitrificans by two-dimensional electrophoresis (cs)
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| skos:prefLabel
| - Analysis of membrane proteome of Paracoccus denitrificans by two-dimensional electrophoresis
- Analysis of membrane proteome of Paracoccus denitrificans by two-dimensional electrophoresis (en)
- Analysis of membrane proteome of Paracoccus denitrificans by two-dimensional electrophoresis (cs)
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| skos:notation
| - RIV/00216224:14310/02:00006413!RIV08-GA0-14310___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14310/02:00006413
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Paracoccus denitrificans; membrane proteins; proteome; 2-D electrophoresis (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
| - Vysoké Tatry, Stará Lesná 10.-13.9.2002
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - XVIII. biochemický zjazd. Zborník.
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Bouchal, Pavel
- Kučera, Igor
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open.../riv/zahajeniAkce
| |
| number of pages
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| http://purl.org/ne...btex#hasPublisher
| - Slovenská spoločnosť pre biochémiu a molekulárnu biológiu pri SAV
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| http://localhost/t...ganizacniJednotka
| |
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