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| rdf:type
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| Description
| - Iron is a component of a number of biological systems, e.g. electron transport chains, cofactors of enzymes, regulatory proteins etc. It occurs in ferric form in environment, while organisms require ferrous iron. Enzymes catalysing the reduction of ferric complexes are widely spread within bacterial kingdom. Some of these enzymes are associated with cytoplasmic membrane, some are present in soluble form in cytoplasm or periplasm, some are excreted into extracellular medium. In our work, cellular fractions of Paracoccus denitrificans were tested for their activity towards reduction of various ferric complexes with NADH as an electron donor. We found the most of the ferric reductase activity in cytosolic fraction. Fe(III)-nitrilotriacetate was chosen as the best artificial substrate for activity measurements. Two proteins responsible for the activity was identified. Both enzymes were purified to homogeneity by combination of FPLC ion-exchange chromatography and chromatofocusing, and characterised with r
- Iron is a component of a number of biological systems, e.g. electron transport chains, cofactors of enzymes, regulatory proteins etc. It occurs in ferric form in environment, while organisms require ferrous iron. Enzymes catalysing the reduction of ferric complexes are widely spread within bacterial kingdom. Some of these enzymes are associated with cytoplasmic membrane, some are present in soluble form in cytoplasm or periplasm, some are excreted into extracellular medium. In our work, cellular fractions of Paracoccus denitrificans were tested for their activity towards reduction of various ferric complexes with NADH as an electron donor. We found the most of the ferric reductase activity in cytosolic fraction. Fe(III)-nitrilotriacetate was chosen as the best artificial substrate for activity measurements. Two proteins responsible for the activity was identified. Both enzymes were purified to homogeneity by combination of FPLC ion-exchange chromatography and chromatofocusing, and characterised with r (en)
- Iron is a component of a number of biological systems, e.g. electron transport chains, cofactors of enzymes, regulatory proteins etc. It occurs in ferric form in environment, while organisms require ferrous iron. Enzymes catalysing the reduction of ferric complexes are widely spread within bacterial kingdom. Some of these enzymes are associated with cytoplasmic membrane, some are present in soluble form in cytoplasm or periplasm, some are excreted into extracellular medium. In our work, cellular fractions of Paracoccus denitrificans were tested for their activity towards reduction of various ferric complexes with NADH as an electron donor. We found the most of the ferric reductase activity in cytosolic fraction. Fe(III)-nitrilotriacetate was chosen as the best artificial substrate for activity measurements. Two proteins responsible for the activity was identified. Both enzymes were purified to homogeneity by combination of FPLC ion-exchange chromatography and chromatofocusing, and characterised with r (cs)
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| Title
| - Purification and characterization of ferric reductases from Paracoccus denitrificans
- Purification and characterization of ferric reductases from Paracoccus denitrificans (en)
- Purification and characterization of ferric reductases from Paracoccus denitrificans (cs)
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| skos:prefLabel
| - Purification and characterization of ferric reductases from Paracoccus denitrificans
- Purification and characterization of ferric reductases from Paracoccus denitrificans (en)
- Purification and characterization of ferric reductases from Paracoccus denitrificans (cs)
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| skos:notation
| - RIV/00216224:14310/02:00006390!RIV08-GA0-14310___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14310/02:00006390
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - ferric reductase; Paracoccus denitrificans; purification (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
| - 10.-13.9.2002 Stará Lesná, Slovenská republika
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Kučera, Igor
- Mazoch, Jiří
- Turánek, Jaroslav
- Tesařík, Radek
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open.../riv/zahajeniAkce
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| number of pages
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| http://purl.org/ne...btex#hasPublisher
| - Slovenská spoločnosť pre biochémiu a molekulárnu biológiu pri SAV
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| http://localhost/t...ganizacniJednotka
| |
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