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| Description
| - This report describes the application of electrophoretically mediated microanalysis (EMMA) for the study of kinetic parameters of bi-substrate enzymatic reaction of rhodanese. The Michaelis constants (Km) for both substrates and the effect of temperature on rhodanese reaction were evaluated by means of the combination of the EMMA methodology with a partial filling technique. In this set-up the part of the capillary is filled with the buffer best for the enzymatic reaction whereas the rest of the capillary with the background electrolyte optimal for separation of substrates and products. The enzymatic reaction was performed in 25 mM HEPES buffer (pH 8.5) while the low pH background electrolyte 100 mM beta-alanine - HCl (pH 3.5) was used for separation of substrates and products that are the inorganic anions. The estimated value of Km for thiosulfate of 1.30 x 10-2 M was consistent with previously published values; the Km for cyanide of 7.6 x 10-3 M was determined for the first time. In addition the typ
- This report describes the application of electrophoretically mediated microanalysis (EMMA) for the study of kinetic parameters of bi-substrate enzymatic reaction of rhodanese. The Michaelis constants (Km) for both substrates and the effect of temperature on rhodanese reaction were evaluated by means of the combination of the EMMA methodology with a partial filling technique. In this set-up the part of the capillary is filled with the buffer best for the enzymatic reaction whereas the rest of the capillary with the background electrolyte optimal for separation of substrates and products. The enzymatic reaction was performed in 25 mM HEPES buffer (pH 8.5) while the low pH background electrolyte 100 mM beta-alanine - HCl (pH 3.5) was used for separation of substrates and products that are the inorganic anions. The estimated value of Km for thiosulfate of 1.30 x 10-2 M was consistent with previously published values; the Km for cyanide of 7.6 x 10-3 M was determined for the first time. In addition the typ (en)
- This report describes the application of electrophoretically mediated microanalysis (EMMA) for the study of kinetic parameters of bi-substrate enzymatic reaction of rhodanese. The Michaelis constants (Km) for both substrates and the effect of temperature on rhodanese reaction were evaluated by means of the combination of the EMMA methodology with a partial filling technique. In this set-up the part of the capillary is filled with the buffer best for the enzymatic reaction whereas the rest of the capillary with the background electrolyte optimal for separation of substrates and products. The enzymatic reaction was performed in 25 mM HEPES buffer (pH 8.5) while the low pH background electrolyte 100 mM beta-alanine - HCl (pH 3.5) was used for separation of substrates and products that are the inorganic anions. The estimated value of Km for thiosulfate of 1.30 x 10-2 M was consistent with previously published values; the Km for cyanide of 7.6 x 10-3 M was determined for the first time. In addition the typ (cs)
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| Title
| - Determination of the kinetic parameters of rhodanese by electrophoretically mediated microanalysis in a partially filled capillary
- Determination of the kinetic parameters of rhodanese by electrophoretically mediated microanalysis in a partially filled capillary (en)
- Determination of the kinetic parameters of rhodanese by electrophoretically mediated microanalysis in a partially filled capillary (cs)
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| skos:prefLabel
| - Determination of the kinetic parameters of rhodanese by electrophoretically mediated microanalysis in a partially filled capillary
- Determination of the kinetic parameters of rhodanese by electrophoretically mediated microanalysis in a partially filled capillary (en)
- Determination of the kinetic parameters of rhodanese by electrophoretically mediated microanalysis in a partially filled capillary (cs)
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| skos:notation
| - RIV/00216224:14310/02:00005658!RIV09-GA0-14310___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14310/02:00005658
|
| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
|
| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Glatz, Zdeněk
- Vytisková, Soňa
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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