About: Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres

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About: Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Magnetic hydrogel microsheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate and ethylene dimethacrylate in the presence of magnetite, which formed the core of the particles. RNase A was coupled to the particles by the cyanuric chloride method. Gel electrophoresis of plasmid DNA pUC19 (contaminated by bacterial RNA) confirmed RNA degradation with the immobilized enzyme. The effect of temperature and pH on the relative activity of immobilized RNase A was estimated after incubation of the samples at different temperatures (30-80 C) and pH (4.0-8.0). Maximum relative activity was observed at 70 C and pH 6.5. The matrice based on magnetic poly(HEMA) had a low tendency to adsorb RNA. Magnetic hydrogel microsheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate and ethylene dimethacrylate in the presence of magnetite, which formed the core of the particles. RNase A was coupled to the particles by the cyanuric chloride method. Gel electrophoresis of plasmid DNA pUC19 (contaminated by bacterial RNA) confirmed RNA degradation with the immobilized enzyme. The effect of temperature and pH on the relative activity of immobilized RNase A was estimated after incubation of the samples at different temperatures (30-80 C) and pH (4.0-8.0). Maximum relative activity was observed at 70 C and pH 6.5. The matrice based on magnetic poly(HEMA) had a low tendency to adsorb RNA. (en) Magnetic hydrogel microsheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate and ethylene dimethacrylate in the presence of magnetite, which formed the core of the particles. RNase A was coupled to the particles by the cyanuric chloride method. Gel electrophoresis of plasmid DNA pUC19 (contaminated by bacterial RNA) confirmed RNA degradation with the immobilized enzyme. The effect of temperature and pH on the relative activity of immobilized RNase A was estimated after incubation of the samples at different temperatures (30-80 C) and pH (4.0-8.0). Maximum relative activity was observed at 70 C and pH 6.5. The matrice based on magnetic poly(HEMA) had a low tendency to adsorb RNA. (cs)
Title	Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres (en) Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres (cs)
skos:prefLabel	Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres (en) Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres (cs)
skos:notation	RIV/00216224:14310/01:00004811!RIV08-GA0-14310___
http://linked.open.../vavai/riv/strany	447
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/00/1339), P(KSK2050602), Z(AV0Z4050913)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Španová, Alena Rittich, Bohuslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	693405
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/01:00004811
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Dispersion polymerization; RNase A; nucleic acids; particles; polystyrene; chromatography; purification (en)
http://linked.open.../riv/klicoveSlovo	chromatography Dispersion polymerization RNase A nucleic acids particles purification polystyrene
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[4EF8F6ACF075]
http://linked.open...i/riv/nazevZdroje	Biotechnology Progress
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Dispersion polymerisation of hydrophilic monomers for the preparation of magnetic carriers used in biotechnological application Targeted synthesis of chemical compounds with defined properties
http://linked.open...UplatneniVysledku	2001
http://linked.open...v/svazekPeriodika	17
http://linked.open...iv/tvurceVysledku	Horák, Daniel Rittich, Bohuslav Šafář, Jan Španová, Alena Beneš, Milan J. Lenfeld, Jiří
http://linked.open...n/vavai/riv/zamer	Cílená syntéza a studium chemických, fyzikálně-chemických a fyzikálních vlastností makromolekulárních a nadmolekulárních látek a soustav pro vyspělé technologie
issn	8756-7938
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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