About: Identification of <I>Staphylococcus aureus</I> based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb <I>Sma</I>I restriction fragment     Goto   Sponge   NotDistinct   Permalink

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Description
  • Primers were designed for polymerase chain reaction (PCR)-amplification of a genomic sequence specific to Staphylococcus aureus strains. The sequence corresponds to a part of the 44-kb Smal fragment (fragment L on the S. aureus NCTC 8325 restriction map) which was found to be common to strains of the S. aureus species (Pantucek et al., 1996, International journal of Systematic Bacteriology, 46: 216-222). The labelled 44-kb Smal restriction fragment derived from S. aureus NCTC 8325-4 was hybridized to the EcoRI restriction patterns of genomic DNA from 13 strains representing different macrorestriction types of S. aureus subsp. aureus. This made it possible to reveal the 2052 bp EcoRI restriction subfragment and to demonstrate its presence in all the tested strains. From the sequence of this subfragment, primers were designed by means of which the 826 bp amplicons were obtained in all 216 tested strains of S. aureus. No hybridization and PCR-products were observed in 40 collection strains of other staph
  • Primers were designed for polymerase chain reaction (PCR)-amplification of a genomic sequence specific to Staphylococcus aureus strains. The sequence corresponds to a part of the 44-kb Smal fragment (fragment L on the S. aureus NCTC 8325 restriction map) which was found to be common to strains of the S. aureus species (Pantucek et al., 1996, International journal of Systematic Bacteriology, 46: 216-222). The labelled 44-kb Smal restriction fragment derived from S. aureus NCTC 8325-4 was hybridized to the EcoRI restriction patterns of genomic DNA from 13 strains representing different macrorestriction types of S. aureus subsp. aureus. This made it possible to reveal the 2052 bp EcoRI restriction subfragment and to demonstrate its presence in all the tested strains. From the sequence of this subfragment, primers were designed by means of which the 826 bp amplicons were obtained in all 216 tested strains of S. aureus. No hybridization and PCR-products were observed in 40 collection strains of other staph (en)
  • Primers were designed for polymerase chain reaction (PCR)-amplification of a genomic sequence specific to Staphylococcus aureus strains. The sequence corresponds to a part of the 44-kb Smal fragment (fragment L on the S. aureus NCTC 8325 restriction map) which was found to be common to strains of the S. aureus species (Pantucek et al., 1996, International journal of Systematic Bacteriology, 46: 216-222). The labelled 44-kb Smal restriction fragment derived from S. aureus NCTC 8325-4 was hybridized to the EcoRI restriction patterns of genomic DNA from 13 strains representing different macrorestriction types of S. aureus subsp. aureus. This made it possible to reveal the 2052 bp EcoRI restriction subfragment and to demonstrate its presence in all the tested strains. From the sequence of this subfragment, primers were designed by means of which the 826 bp amplicons were obtained in all 216 tested strains of S. aureus. No hybridization and PCR-products were observed in 40 collection strains of other staph (cs)
Title
  • Identification of <I>Staphylococcus aureus</I> based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb <I>Sma</I>I restriction fragment
  • Identification of <I>Staphylococcus aureus</I> based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb <I>Sma</I>I restriction fragment (en)
  • Identification of <I>Staphylococcus aureus</I> based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb <I>Sma</I>I restriction fragment (cs)
skos:prefLabel
  • Identification of <I>Staphylococcus aureus</I> based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb <I>Sma</I>I restriction fragment
  • Identification of <I>Staphylococcus aureus</I> based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb <I>Sma</I>I restriction fragment (en)
  • Identification of <I>Staphylococcus aureus</I> based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb <I>Sma</I>I restriction fragment (cs)
skos:notation
  • RIV/00216224:14310/01:00004693!RIV08-MSM-14310___
http://linked.open.../vavai/riv/strany
  • 249
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA301/99/D075), Z(MSM 143100008)
http://linked.open...iv/cisloPeriodika
  • 5
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 682279
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/01:00004693
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Staphylococcus aureus; polymerase chain reaction; species-specific probe; 44-kb SmaI fragment L of NCTC 8325; molecular identification (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [28EBC9AB5554]
http://linked.open...i/riv/nazevZdroje
  • Molecular and Cellular Probes
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 15
http://linked.open...iv/tvurceVysledku
  • Doškař, Jiří
  • Pantůček, Roman
  • Růžičková, Vladislava
  • Štěpán, Jiří
  • Hájek, Václav
  • Rosypal, Stanislav
http://linked.open...n/vavai/riv/zamer
issn
  • 0890-8508
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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