About: Identification of Staphylococcus aureus based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb SmaI restriction fragment

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About: Identification of Staphylococcus aureus based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb SmaI restriction fragment Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Primers were designed for polymerase chain reaction (PCR)-amplification of a genomic sequence specific to Staphylococcus aureus strains. The sequence corresponds to a part of the 44-kb Smal fragment (fragment L on the S. aureus NCTC 8325 restriction map) which was found to be common to strains of the S. aureus species (Pantucek et al., 1996, International journal of Systematic Bacteriology, 46: 216-222). The labelled 44-kb Smal restriction fragment derived from S. aureus NCTC 8325-4 was hybridized to the EcoRI restriction patterns of genomic DNA from 13 strains representing different macrorestriction types of S. aureus subsp. aureus. This made it possible to reveal the 2052 bp EcoRI restriction subfragment and to demonstrate its presence in all the tested strains. From the sequence of this subfragment, primers were designed by means of which the 826 bp amplicons were obtained in all 216 tested strains of S. aureus. No hybridization and PCR-products were observed in 40 collection strains of other staph Primers were designed for polymerase chain reaction (PCR)-amplification of a genomic sequence specific to Staphylococcus aureus strains. The sequence corresponds to a part of the 44-kb Smal fragment (fragment L on the S. aureus NCTC 8325 restriction map) which was found to be common to strains of the S. aureus species (Pantucek et al., 1996, International journal of Systematic Bacteriology, 46: 216-222). The labelled 44-kb Smal restriction fragment derived from S. aureus NCTC 8325-4 was hybridized to the EcoRI restriction patterns of genomic DNA from 13 strains representing different macrorestriction types of S. aureus subsp. aureus. This made it possible to reveal the 2052 bp EcoRI restriction subfragment and to demonstrate its presence in all the tested strains. From the sequence of this subfragment, primers were designed by means of which the 826 bp amplicons were obtained in all 216 tested strains of S. aureus. No hybridization and PCR-products were observed in 40 collection strains of other staph (en)
Title	Identification of Staphylococcus aureus based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb SmaI restriction fragment Identification of Staphylococcus aureus based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb SmaI restriction fragment (en)
skos:prefLabel	Identification of Staphylococcus aureus based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb SmaI restriction fragment Identification of Staphylococcus aureus based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb SmaI restriction fragment (en)
skos:notation	RIV/00216224:14310/01:00004693!RIV/2002/MSM/143102/N
http://linked.open.../vavai/riv/strany	249
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/99/D075), Z(MSM 143100008)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Štěpán, Jiří Rosypal, Stanislav Doškař, Jiří Růžičková, Vladislava Pantůček, Roman
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	682295
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/01:00004693
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Staphylococcus aureus, polymerase chain reaction, species-specific probe, 44-kb SmaI fragment L of NCTC 8325, molecular identification (en)
http://linked.open.../riv/klicoveSlovo	polymerase chain reaction molecular identification Staphylococcus aureus 44-kb SmaI fragment L of NCTC 8325 species-specific probe
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[881FBEDD34A5]
http://linked.open...i/riv/nazevZdroje	Molecular and Cellular Probes
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Genome-based diagnostics and typing of coagulase-negative staphylococci from human clinical specimens
http://linked.open...UplatneniVysledku	2001
http://linked.open...v/svazekPeriodika	15
http://linked.open...iv/tvurceVysledku	Doškař, Jiří Pantůček, Roman Růžičková, Vladislava Štěpán, Jiří Hájek, Václav Rosypal, Stanislav
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20143100008
issn	0890-8508
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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