About: Genetic characterization of six species of diplozoids (Monogenea, Diplozoidae)     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

AttributesValues
rdf:type
Description
  • The second internal transcribed spacer (ITS2)of the ribosomal RNA gene array of 6 species of diplozoids was amplified by PCR and sequenced. These sequences clearly demonstrate discrimination at the species level and confirm the validity of species determined by morphological identification. No intraspecific variation was found in the ITS2 sequences. There were no differences in the ITS2 sequenced of P. homoion from populations parasitizing different host species. The length of the PCR product allowed discrimination of E. nipponicum from the Paradiplozoon species. Digestion of the amplified ITS2 fragment with enzymes AluI, HaeIII and HifI provided useful genetic markers for species identification. This represent the first molecular taxonomic study of these interesting parasites and demonstrates the utility of these methods for addressing questions of systematics.
  • The second internal transcribed spacer (ITS2)of the ribosomal RNA gene array of 6 species of diplozoids was amplified by PCR and sequenced. These sequences clearly demonstrate discrimination at the species level and confirm the validity of species determined by morphological identification. No intraspecific variation was found in the ITS2 sequences. There were no differences in the ITS2 sequenced of P. homoion from populations parasitizing different host species. The length of the PCR product allowed discrimination of E. nipponicum from the Paradiplozoon species. Digestion of the amplified ITS2 fragment with enzymes AluI, HaeIII and HifI provided useful genetic markers for species identification. This represent the first molecular taxonomic study of these interesting parasites and demonstrates the utility of these methods for addressing questions of systematics. (en)
Title
  • Genetic characterization of six species of diplozoids (Monogenea, Diplozoidae)
  • Genetic characterization of six species of diplozoids (Monogenea, Diplozoidae) (en)
skos:prefLabel
  • Genetic characterization of six species of diplozoids (Monogenea, Diplozoidae)
  • Genetic characterization of six species of diplozoids (Monogenea, Diplozoidae) (en)
skos:notation
  • RIV/00216224:14310/01:00004340!RIV/2002/MSM/143102/N
http://linked.open.../vavai/riv/strany
  • 465
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM 143100010)
http://linked.open...iv/cisloPeriodika
  • -
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 681048
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/01:00004340
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Monogenea, Diplozoidae, PCR, ITS2, RFLP, genetic distance (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [0CA201F2344B]
http://linked.open...i/riv/nazevZdroje
  • Parasitology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...ocetUcastnikuAkce
http://linked.open...nichUcastnikuAkce
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 123
http://linked.open...iv/tvurceVysledku
  • Koubková, Božena
  • Matějusová, Iveta
  • Cunningham, Carey O.
  • D'Amelio, Stefano
http://linked.open...n/vavai/riv/zamer
issn
  • 0031-1820
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
Faceted Search & Find service v1.16.118 as of Jun 21 2024


Alternative Linked Data Documents: ODE     Content Formats:   [cxml] [csv]     RDF   [text] [turtle] [ld+json] [rdf+json] [rdf+xml]     ODATA   [atom+xml] [odata+json]     Microdata   [microdata+json] [html]    About   
This material is Open Knowledge   W3C Semantic Web Technology [RDF Data] Valid XHTML + RDFa
OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software