About: Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei)

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About: Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei) Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Thirty-one gyrodactylid species from five families of freshwater fish were examined and variable region V4 of the 18S small subunit ribosomal RNA gene and ribosomal RNA internal transcribed spacers ITS1 and ITS2 were sequenced. Both the V4 region and spacers ITS1 and ITS2 proved useful for gyrodactylid diagnosis. Sequences of these fragments exhibited interspecific variations and allowed clear determination at the species level. In some cases, the length of the ITS1 PCR fragment provided useful genetic markers. Species that yielded a short ITS1 fragment also showed distinct groupings in ITS2 and V4 sequences that were markedly different to sequences from species that contain a long ITS1. Repetitive sequences located in the ITS1 of Gyrodactylus gobii and Gyrodactylus vimbi accounted for some of the variations in length of PCR products. There was no evidence for intraspecific variation within these regions and short tandem repeats were not found in the other species studied. The number of polymorphic an Thirty-one gyrodactylid species from five families of freshwater fish were examined and variable region V4 of the 18S small subunit ribosomal RNA gene and ribosomal RNA internal transcribed spacers ITS1 and ITS2 were sequenced. Both the V4 region and spacers ITS1 and ITS2 proved useful for gyrodactylid diagnosis. Sequences of these fragments exhibited interspecific variations and allowed clear determination at the species level. In some cases, the length of the ITS1 PCR fragment provided useful genetic markers. Species that yielded a short ITS1 fragment also showed distinct groupings in ITS2 and V4 sequences that were markedly different to sequences from species that contain a long ITS1. Repetitive sequences located in the ITS1 of Gyrodactylus gobii and Gyrodactylus vimbi accounted for some of the variations in length of PCR products. There was no evidence for intraspecific variation within these regions and short tandem repeats were not found in the other species studied. The number of polymorphic an (en)
Title	Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei) Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei) (en)
skos:prefLabel	Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei) Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei) (en)
skos:notation	RIV/00216224:14310/01:00004169!RIV/2002/MSM/143102/N
http://linked.open.../vavai/riv/strany	738
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA524/00/0844), P(GA524/98/0940), Z(MSM 143100010)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Gelnar, Milan Matějusová, Iveta
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	687575
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/01:00004169
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Gyrodactylus; rRNA; Polymerase chain reaction; Internal transcribed spacers; species-level identification (en)
http://linked.open.../riv/klicoveSlovo	Gyrodactylus Polymerase chain reaction rRNA Internal transcribed spacers species-level identification
http://linked.open...odStatuVydavatele	ID - Indonéská republika
http://linked.open...ontrolniKodProRIV	[D3C49095336D]
http://linked.open...i/riv/nazevZdroje	International Journal for Parasitology
http://linked.open...in/vavai/riv/obor	EG
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Neurobiology, surface architecture and microhabitat segregation of model parasite species during ontogenic development Diversity of parasites like an indicator of environmental stress
http://linked.open...UplatneniVysledku	2001
http://linked.open...v/svazekPeriodika	31
http://linked.open...iv/tvurceVysledku	Gelnar, Milan Matějusová, Iveta Cunningham, Carey O. Collins, Catherine M. McBeath, Alastair J. A.
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20143100010
issn	0020-7519
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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