About: Stanovení kinetických parametrů rhodanasy pomoci metody EMMA (Electrophoretivally Mediated Microanalysis)

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Stanovení kinetických parametrů rhodanasy pomoci metody EMMA (Electrophoretivally Mediated Microanalysis) Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Recently, a new application for the evaluation of enzymatic reactions in capillary electrophoresi was proposed, electrophoretically mediated microanalysis (EMMA). In this technique, substrate(s) and enzyme are introduced in the capillary as distinct plugs, the first analyte injected being the one with the lower electrophoretic mobility. Upon the application of an electric field, the two zones interpenetrate due the differences in their electrophoretic mobilities. Enzymatic reaction takes place and the resultant reaction product(s) and the unreacted substrate(s) are electrophoretically transported towards detector, where they are individually detected. In this paper the EMMA technique was applied to determine the kinetic parameters of rhodanese. Rhodanese (thiosulfate: cyanide sulfur transferase, EC 2.8.1.1) was discovered in 1933 by Lang. It is responsible for the transfer of the sulfane sulfur of thiosulfate to an acceptor, which is likely to be cyanide under some physiological conditions. Rhodane Recently, a new application for the evaluation of enzymatic reactions in capillary electrophoresi was proposed, electrophoretically mediated microanalysis (EMMA). In this technique, substrate(s) and enzyme are introduced in the capillary as distinct plugs, the first analyte injected being the one with the lower electrophoretic mobility. Upon the application of an electric field, the two zones interpenetrate due the differences in their electrophoretic mobilities. Enzymatic reaction takes place and the resultant reaction product(s) and the unreacted substrate(s) are electrophoretically transported towards detector, where they are individually detected. In this paper the EMMA technique was applied to determine the kinetic parameters of rhodanese. Rhodanese (thiosulfate: cyanide sulfur transferase, EC 2.8.1.1) was discovered in 1933 by Lang. It is responsible for the transfer of the sulfane sulfur of thiosulfate to an acceptor, which is likely to be cyanide under some physiological conditions. Rhodane (en)
Title	Stanovení kinetických parametrů rhodanasy pomoci metody EMMA (Electrophoretivally Mediated Microanalysis) Stanovení kinetických parametrů rhodanasy pomoci metody EMMA (Electrophoretivally Mediated Microanalysis) (en)
skos:prefLabel	Stanovení kinetických parametrů rhodanasy pomoci metody EMMA (Electrophoretivally Mediated Microanalysis) Stanovení kinetických parametrů rhodanasy pomoci metody EMMA (Electrophoretivally Mediated Microanalysis) (en)
skos:notation	RIV/00216224:14310/01:00003600!RIV/2002/GA0/143102/N
http://linked.open.../vavai/riv/strany	48
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA525/00/0785)
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Vytisková, Soňa Glatz, Zdeněk
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	696993
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/01:00003600
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Rhodanese, Electrophoretivally Mediated Microanalysis (en)
http://linked.open.../riv/klicoveSlovo	Rhodanese Electrophoretivally Mediated Microanalysis
http://linked.open...ontrolniKodProRIV	[4341EF293F9D]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	Sborník příspěvků 5.Pracovního setkání biochemiků a molekulárních biologů
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Acidification processes in sulphide wastes
http://linked.open...UplatneniVysledku	2001
http://linked.open...iv/tvurceVysledku	Glatz, Zdeněk Nováková, Soňa
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2001-01-01 (xsd:date)
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Masarykova univerzita v Brně. Přírodovědecká fakulta
https://schema.org/isbn	80-210-2538-7
http://localhost/t...ganizacniJednotka	14310

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software