About: Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60

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About: Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60 Goto Sponge NotDistinct Permalink

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Description	Tumor necrosis factor-a (TNF-a) has been found to reduce cellular proliferation and viability, and to induce apoptosis, as well as a partial differentiation. These effects could contribute to elimination of leukemic cells. Using human leukemic cell line HL-60 as a model, we administered cyclooxygenase (indomethacin) and lipoxygenase (MK-886) inhibitors together with TNF-a, in order to evaluate the possible role of prostaglandins and leukotrienes in these processes. The following methods were used to assess cell proliferation, differentiation and apoptosis: cells were counted with haemocytometer; apoptosis and cell cycle were quantified using flowcytometry and fluorescence microscopy; viability was detected by means of light microscopy and flowcytometry; differentiation was followed as nonspecific esterase activity. Our results indicate that indomethacin can potentiate TNF-a-induced apoptosis, resulting in the decrease of number of cells and their viability during simultaneous treatment with TNF-a. Tumor necrosis factor-a (TNF-a) has been found to reduce cellular proliferation and viability, and to induce apoptosis, as well as a partial differentiation. These effects could contribute to elimination of leukemic cells. Using human leukemic cell line HL-60 as a model, we administered cyclooxygenase (indomethacin) and lipoxygenase (MK-886) inhibitors together with TNF-a, in order to evaluate the possible role of prostaglandins and leukotrienes in these processes. The following methods were used to assess cell proliferation, differentiation and apoptosis: cells were counted with haemocytometer; apoptosis and cell cycle were quantified using flowcytometry and fluorescence microscopy; viability was detected by means of light microscopy and flowcytometry; differentiation was followed as nonspecific esterase activity. Our results indicate that indomethacin can potentiate TNF-a-induced apoptosis, resulting in the decrease of number of cells and their viability during simultaneous treatment with TNF-a. (en)
Title	Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60 Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60 (en)
skos:prefLabel	Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60 Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60 (en)
skos:notation	RIV/00216224:14310/00:00003964!RIV/2002/GA0/143102/N
http://linked.open.../vavai/riv/strany	135
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA524/99/0694)
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Štika, Jiří Šimek, Vladimír
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	725580
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/00:00003964
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60 (en)
http://linked.open.../riv/klicoveSlovo	Viability Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60 Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation
http://linked.open...ontrolniKodProRIV	[9C71C82709CB]
http://linked.open...v/mistoKonaniAkce	České Budějovice
http://linked.open...i/riv/mistoVydani	České Budějovice
http://linked.open...i/riv/nazevZdroje	Cells II
http://linked.open...in/vavai/riv/obor	EC
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http://linked.open...vavai/riv/projekt	Polyunsaturated fatty acids and cytokines - their role in maintenance of homeostasis at the cell population level
http://linked.open...UplatneniVysledku	2000
http://linked.open...iv/tvurceVysledku	Hofmanová, Jiřina Kozubík, Alois Vondráček, Jan Štika, Jiří Šimek, Vladimír
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2000-01-01 (xsd:date)
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