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rdf:type
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Description
| - A bioactive sorbent for degradation of high-molecular-weight RNA by RNase A was prepared by immobilization of RNase A on magnetic spherical carriers based on cellulose or poly(HEMA-co-EDMA). Immobilized RNase A was temperature-stable at 30-60 C. The influence of temperature on immobilized enzyme activity was also studied. A comparison of soluble and immobilized RNase A showed a pH shift in maximum of immobilized enzyme activity in dependence on the matrix type. Practical use of RNase A immobilized on magnetic bead cellulose MBC 1 for high-performance size-exclusion chromatography purification of pUC19 plasmid DNA is demonstrated.
- A bioactive sorbent for degradation of high-molecular-weight RNA by RNase A was prepared by immobilization of RNase A on magnetic spherical carriers based on cellulose or poly(HEMA-co-EDMA). Immobilized RNase A was temperature-stable at 30-60 C. The influence of temperature on immobilized enzyme activity was also studied. A comparison of soluble and immobilized RNase A showed a pH shift in maximum of immobilized enzyme activity in dependence on the matrix type. Practical use of RNase A immobilized on magnetic bead cellulose MBC 1 for high-performance size-exclusion chromatography purification of pUC19 plasmid DNA is demonstrated. (en)
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Title
| - Characterisation of Ribonuclease A Immobilized on Magnetic Cellulose - and Poly(2-Hydroxyethyl Methacrylate)-Based Supports
- Characterisation of Ribonuclease A Immobilized on Magnetic Cellulose - and Poly(2-Hydroxyethyl Methacrylate)-Based Supports (en)
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skos:prefLabel
| - Characterisation of Ribonuclease A Immobilized on Magnetic Cellulose - and Poly(2-Hydroxyethyl Methacrylate)-Based Supports
- Characterisation of Ribonuclease A Immobilized on Magnetic Cellulose - and Poly(2-Hydroxyethyl Methacrylate)-Based Supports (en)
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skos:notation
| - RIV/00216224:14310/00:00003595!RIV/2002/GA0/143102/N
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14310/00:00003595
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - immobilized RNase A, magnetic bead cellulose, magnetic poly(HEMA-co-EDMA) microspheres, temperature stability, pH stability, plasmid purification (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - International Journal of Bio-Chromatography
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...ocetUcastnikuAkce
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http://linked.open...nichUcastnikuAkce
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Horák, Daniel
- Rittich, Bohuslav
- Šafář, Jan
- Španová, Alena
- Lenfeld, Jiří
- Beneš, Milan
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number of pages
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http://localhost/t...ganizacniJednotka
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