About: Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2-dipyridyldisulphide

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2-dipyridyldisulphide Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A new method for specific determination of glutathione using micellar electrokinetic chromatography and on-column reaction with 2,2 -dipyridyldisulphide is described. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively into the capillary as two discrete plugs separated with a short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione, on-column mixing and reaction occur. Glutathione is in this reaction quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amount of the 2-thiopyridone which is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of glutathione is thus estimated indirectly from the result of 2-thiopyridone determination. A new method for specific determination of glutathione using micellar electrokinetic chromatography and on-column reaction with 2,2 -dipyridyldisulphide is described. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively into the capillary as two discrete plugs separated with a short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione, on-column mixing and reaction occur. Glutathione is in this reaction quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amount of the 2-thiopyridone which is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of glutathione is thus estimated indirectly from the result of 2-thiopyridone determination. (en)
Title	Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2-dipyridyldisulphide Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2-dipyridyldisulphide (en)
skos:prefLabel	Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2-dipyridyldisulphide Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2-dipyridyldisulphide (en)
skos:notation	RIV/00216224:14310/00:00003314!RIV/2002/GA0/143102/N
http://linked.open.../vavai/riv/strany	179
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA525/00/0785)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Mašlaňová, Hana Glatz, Zdeněk
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	727276
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/00:00003314
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	thiol, on-column derivatization, MEKC (en)
http://linked.open.../riv/klicoveSlovo	MEKC thiol on-column derivatization
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[6AD78B778534]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Acidification processes in sulphide wastes
http://linked.open...UplatneniVysledku	2000
http://linked.open...v/svazekPeriodika	895
http://linked.open...iv/tvurceVysledku	Glatz, Zdeněk Mašlaňová, Hana
issn	0021-9606
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software