About: Telomere-binding Proteins from Nuclei of Telomerase-Negative Tissues Inhibit Plant Telomerase (invited lecture)     Goto   Sponge   NotDistinct   Permalink

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  • The activity of telomerase in plant cells is precisely regulated in response to changes in cell division rate. To explore this regulatory mechanism, the effect on telomerase activity of protein extracts from nuclei of telomerase-negative tissues was examined. An inhibition of telomerase activity was found which was species-non-specific. This inhibition was due to proteins which form salt-stable, sequence- specific complexes with the G-rich telomeric strand and reduce its accessibility, as shown by gel retardation and by terminal transferase (TdT) extension of G-rich telomeric and non-telomeric (substrate) primers. A 40 kDa polypeptide was detected by SDS-PAGE after cross- linking the complex formed by extracts from tobacco leaf nuclei. Such proteins may be involved in regulation of telomerase activity in plants.
  • The activity of telomerase in plant cells is precisely regulated in response to changes in cell division rate. To explore this regulatory mechanism, the effect on telomerase activity of protein extracts from nuclei of telomerase-negative tissues was examined. An inhibition of telomerase activity was found which was species-non-specific. This inhibition was due to proteins which form salt-stable, sequence- specific complexes with the G-rich telomeric strand and reduce its accessibility, as shown by gel retardation and by terminal transferase (TdT) extension of G-rich telomeric and non-telomeric (substrate) primers. A 40 kDa polypeptide was detected by SDS-PAGE after cross- linking the complex formed by extracts from tobacco leaf nuclei. Such proteins may be involved in regulation of telomerase activity in plants. (en)
  • The activity of telomerase in plant cells is precisely regulated in response to changes in cell division rate. To explore this regulatory mechanism, the effect on telomerase activity of protein extracts from nuclei of telomerase-negative tissues was examined. An inhibition of telomerase activity was found which was species-non-specific. This inhibition was due to proteins which form salt-stable, sequence- specific complexes with the G-rich telomeric strand and reduce its accessibility, as shown by gel retardation and by terminal transferase (TdT) extension of G-rich telomeric and non-telomeric (substrate) primers. A 40 kDa polypeptide was detected by SDS-PAGE after cross- linking the complex formed by extracts from tobacco leaf nuclei. Such proteins may be involved in regulation of telomerase activity in plants. (cs)
Title
  • Telomere-binding Proteins from Nuclei of Telomerase-Negative Tissues Inhibit Plant Telomerase (invited lecture)
  • Telomere-binding Proteins from Nuclei of Telomerase-Negative Tissues Inhibit Plant Telomerase (invited lecture) (en)
  • Telomere-binding Proteins from Nuclei of Telomerase-Negative Tissues Inhibit Plant Telomerase (invited lecture) (cs)
skos:prefLabel
  • Telomere-binding Proteins from Nuclei of Telomerase-Negative Tissues Inhibit Plant Telomerase (invited lecture)
  • Telomere-binding Proteins from Nuclei of Telomerase-Negative Tissues Inhibit Plant Telomerase (invited lecture) (en)
  • Telomere-binding Proteins from Nuclei of Telomerase-Negative Tissues Inhibit Plant Telomerase (invited lecture) (cs)
skos:notation
  • RIV/00216224:14310/00:00002418!RIV08-MSM-14310___
http://linked.open.../vavai/riv/strany
  • 1132
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(VS97032), Z(MSM 143100008)
http://linked.open...iv/cisloPeriodika
  • 6
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...dnocenehoVysledku
  • 728988
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/00:00002418
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Telomere-binding Proteins (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [FB64174A0F5D]
http://linked.open...i/riv/nazevZdroje
  • J.Biomol. Struct. Dyn.
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 17
http://linked.open...iv/tvurceVysledku
  • Fajkus, Jiří
  • Fulnečková, Jana
http://linked.open...n/vavai/riv/zamer
issn
  • 0739-1102
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  • 14310
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