About: Preparation of a speciesspecific molecular probe for identification of Staphylococcus aureus

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In our previous work a 44-kb genomic SmaI-restriction fragment common to all S. aureus strains has been described. This restriction fragment was used as a starting point in preparation of a species-specific hybridisation probe for rapid molecular identification S. aureus strains. EcoRI-restricted genomic DNAs isolated from 13 different S. aureus strains, including the type strain S. aureus CCM 885, and from the type strain S. epidermidis CCM 2124 (negative control) were hybridised to a probe prepared from the whole sequence of the 44-kb fragment. The EcoRI-restriction fragments showing strong hybridisation to the S. aureus 44-kb probe were cloned in the pUCl8 vector in E. coli and used as candidates for the preparation of molecular probes specific for S. aureus. The cloned 2-kb EcoRI-restriction fragment was chosen as the most specific fragment that hybridised to the homologic EcoRl-fragment present in genomic DNA of more than 100 S. aureus strains of different provenance. No hybridisation was detecte In our previous work a 44-kb genomic SmaI-restriction fragment common to all S. aureus strains has been described. This restriction fragment was used as a starting point in preparation of a species-specific hybridisation probe for rapid molecular identification S. aureus strains. EcoRI-restricted genomic DNAs isolated from 13 different S. aureus strains, including the type strain S. aureus CCM 885, and from the type strain S. epidermidis CCM 2124 (negative control) were hybridised to a probe prepared from the whole sequence of the 44-kb fragment. The EcoRI-restriction fragments showing strong hybridisation to the S. aureus 44-kb probe were cloned in the pUCl8 vector in E. coli and used as candidates for the preparation of molecular probes specific for S. aureus. The cloned 2-kb EcoRI-restriction fragment was chosen as the most specific fragment that hybridised to the homologic EcoRl-fragment present in genomic DNA of more than 100 S. aureus strains of different provenance. No hybridisation was detecte (en)
Title	Preparation of a speciesspecific molecular probe for identification of Staphylococcus aureus Preparation of a speciesspecific molecular probe for identification of Staphylococcus aureus (en)
skos:prefLabel	Preparation of a speciesspecific molecular probe for identification of Staphylococcus aureus Preparation of a speciesspecific molecular probe for identification of Staphylococcus aureus (en)
skos:notation	RIV/00216224:14310/00:00002298!RIV/2002/MSM/143102/N
http://linked.open.../vavai/riv/strany	380
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/99/D075), Z(MSM 143100008)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Štěpán, Jiří Rosypal, Stanislav Doškař, Jiří Růžičková, Vladislava Pantůček, Roman
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	722934
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/00:00002298
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Preparation of a speciesspecific molecular probe for identification of Staphylococcus aureus (en)
http://linked.open.../riv/klicoveSlovo	Preparation of a speciesspecific molecular probe for identification of Staphylococcus aureus
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[295688C7C6E5]
http://linked.open...i/riv/nazevZdroje	Scripta medica
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Genome-based diagnostics and typing of coagulase-negative staphylococci from human clinical specimens
http://linked.open...UplatneniVysledku	2000
http://linked.open...v/svazekPeriodika	72
http://linked.open...iv/tvurceVysledku	Doškař, Jiří Pantůček, Roman Růžičková, Vladislava Štěpán, Jiří Rosypal, Stanislav
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20143100008
issn	1211-3395
number of pages	1 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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