About: Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic

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About: Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this study was to estimate the genetic variability of 21 Staphylococcus epidermidis, 20 Staphylococcus haemolyticus and 30 Staphylococcus hominis strains resistant to oxacillin and multiresistant to antibiotics isolated from various clinical materials in Czech Republic during 1996-1999. Identification of these strains was carried out by estimating their biochemical features by use of STAPHYTest 16 and the ID32 Staph systems. In all the strains the resistance to 15 antibiotics was tested. The phage-typing was performed in using the Lyon and London phage sets at 100xRTD as well as the polyvalent phages and their host-range mutants. Oxacillin resistance was confirmed by the gene mecA PCR-amplification. For purpose of estimating genetic relationships in all the strains under study the SmaI-macrorestriction analysis, ribotyping, plasmid content and PCR-amplification of spacer region in 16S-23S rDNA were carried out. The results of SmaI-macrorestriction analysis in S. epidermidis indicated the The aim of this study was to estimate the genetic variability of 21 Staphylococcus epidermidis, 20 Staphylococcus haemolyticus and 30 Staphylococcus hominis strains resistant to oxacillin and multiresistant to antibiotics isolated from various clinical materials in Czech Republic during 1996-1999. Identification of these strains was carried out by estimating their biochemical features by use of STAPHYTest 16 and the ID32 Staph systems. In all the strains the resistance to 15 antibiotics was tested. The phage-typing was performed in using the Lyon and London phage sets at 100xRTD as well as the polyvalent phages and their host-range mutants. Oxacillin resistance was confirmed by the gene mecA PCR-amplification. For purpose of estimating genetic relationships in all the strains under study the SmaI-macrorestriction analysis, ribotyping, plasmid content and PCR-amplification of spacer region in 16S-23S rDNA were carried out. The results of SmaI-macrorestriction analysis in S. epidermidis indicated the (en)
Title	Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic (en)
skos:prefLabel	Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic (en)
skos:notation	RIV/00216224:14310/00:00002296!RIV/2002/MSM/143102/N
http://linked.open.../vavai/riv/strany	104
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/99/D075), Z(MSM 143100008)
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Rybář, Roman Kailerová, Jana Rosypal, Stanislav Doškař, Jiří Sedláček, Ivo Růžičková, Vladislava Pantůček, Roman Švec, Pavel Ondroušková, Eva Holčáková, Jitka
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	706589
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/00:00002296
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic (en)
http://linked.open.../riv/klicoveSlovo	Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic
http://linked.open...ontrolniKodProRIV	[41EDE58B2E4B]
http://linked.open...v/mistoKonaniAkce	Kolding
http://linked.open...i/riv/mistoVydani	Kolding
http://linked.open...i/riv/nazevZdroje	9th International Symposium on Staphylococci and Staphylococcal Infections
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	10 (xsd:int)
http://linked.open...cetTvurcuVysledku	12 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Genome-based diagnostics and typing of coagulase-negative staphylococci from human clinical specimens
http://linked.open...UplatneniVysledku	2000
http://linked.open...iv/tvurceVysledku	Doškař, Jiří Pantůček, Roman Růžičková, Vladislava Sedláček, Ivo Švec, Pavel Rybář, Roman Petráš, Petr Hájek, Václav Kailerová, Jana Rosypal, Stanislav Faitová, Jitka Zahradníčková, Eva
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2000-01-01 (xsd:date)
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20143100008
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	JDS
http://localhost/t...ganizacniJednotka	14310

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