About: Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids

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About: Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A beta-glucosidase that cleaves the biologically inactive hormone conjugates cytokinin-O- and kinetin-N3-glucosides is encoded by the maize Zm-p60.1 gene. The expression of the Zm-p60.1 gene was analyzed by Northern blot analysis and in-situ hybridization. It was found that the expression levels of the Zm-p60.1-specific mRNA changed after pollination of carpellate inflorescences. The Zm-p60.1 c DNA was expressed in E. coli and antibodies were raised against the protein. An antibody was used to determine the tissue-specific localization of this protein. By in situ immunolocalization experiments, this protein was found to be located in cell layers below the epidermis and around the vascular bundles of the coleoptile. In the primary leaf, the Zm-p60.1 protein was detected in cells of the outermost cell layer and arround the vascular tissue. In floral tissue, Zm-p60.1 was present in the glumes, the carpels and in the outer cell layer of the style. In the coleoptiles, as determined by immuno-electronmicros A beta-glucosidase that cleaves the biologically inactive hormone conjugates cytokinin-O- and kinetin-N3-glucosides is encoded by the maize Zm-p60.1 gene. The expression of the Zm-p60.1 gene was analyzed by Northern blot analysis and in-situ hybridization. It was found that the expression levels of the Zm-p60.1-specific mRNA changed after pollination of carpellate inflorescences. The Zm-p60.1 c DNA was expressed in E. coli and antibodies were raised against the protein. An antibody was used to determine the tissue-specific localization of this protein. By in situ immunolocalization experiments, this protein was found to be located in cell layers below the epidermis and around the vascular bundles of the coleoptile. In the primary leaf, the Zm-p60.1 protein was detected in cells of the outermost cell layer and arround the vascular tissue. In floral tissue, Zm-p60.1 was present in the glumes, the carpels and in the outer cell layer of the style. In the coleoptiles, as determined by immuno-electronmicros (en)
Title	Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids (en)
skos:prefLabel	Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids (en)
skos:notation	RIV/00216224:14310/00:00001867!RIV/2002/MSM/143102/N
http://linked.open.../vavai/riv/strany	407
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ME 040), P(VS96096), Z(AV0Z5004920)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Brzobohatý, Břetislav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	708589
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/00:00001867
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cytokinin - Cytokinin conjugate - <beta>-Glucosidase - Immunolocalization - Zea (en)
http://linked.open.../riv/klicoveSlovo	cytokinin
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[09AF137A460E]
http://linked.open...i/riv/nazevZdroje	Planta
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Laboratory of Molecular Plant Physiology http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20040
http://linked.open...UplatneniVysledku	2000
http://linked.open...v/svazekPeriodika	210
http://linked.open...iv/tvurceVysledku	Brzobohatý, Břetislav Palme, Klaus Bako, Laszlo Höhfeld, Ingo Kristoffersen, Peter Melkonian, Michael
http://linked.open...n/vavai/riv/zamer	Biophysical properties of living systems and their changes under the influence of environmental factors
issn	0032-0935
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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